Comparative effects of fipronil and its metabolites sulfone and desulfinyl on the isolated rat liver mitochondria

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dc.contributor.authorTavares, Marco A. [UNESP]
dc.contributor.authorPalma, Ivo D. F. [UNESP]
dc.contributor.authorMedeiros, Hyllana C. D. [UNESP]
dc.contributor.authorGuelfi, Marieli [UNESP]
dc.contributor.authorSantana, Andréia T. [UNESP]
dc.contributor.authorMingatto, Fábio E. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-12-07T15:35:59Z
dc.date.available2015-12-07T15:35:59Z
dc.date.issued2015
dc.description.abstractFipronil is an insecticide extensively used to control pests in crops and animals. There are relates of poisoning due to exposure of fipronil in mammals and the liver has been suggested as potential target. In this study, we evaluated the effects of fipronil and its metabolites sulfone and desulfinyl on the bioenergetics, reactive oxygen species (ROS) production and calcium efflux from mitochondria isolated from rat liver. Fipronil (5-25 μM) inhibited state-3 respiration in mitochondria energized with glutamate plus malate, substrates of complex I of the respiratory chain and decreased the mitochondrial membrane potential resulting in inhibition of ATP synthesis. Fipronil also caused uncoupling in succinate-energized mitochondria and calcium efflux. The metabolites sulfone and desulfinyl also acted as mitochondrial inhibitors and uncouplers and caused calcium efflux, but with different potencies, being the sulfone the more potent one. These effects of fipronil and its metabolites on mitochondrial bioenergetics and calcium homeostasis may be related to toxic effects of the insecticide in the liver.en
dc.description.affiliationLaboratory of Metabolic and Toxicological Biochemistry (LaBMeT), UNESP - Univ Estadual Paulista, Campus of Dracena, Dracena, SP, Brazil.
dc.description.affiliationLaboratory of Metabolic and Toxicological Biochemistry (LaBMeT), UNESP - Univ Estadual Paulista, Campus of Dracena, Dracena, SP, Brazil. Electronic address: fmingatto@dracena.unesp.br.
dc.description.affiliationUnespLaboratory of Metabolic and Toxicological Biochemistry (LaBMeT), UNESP - Univ Estadual Paulista, Campus of Dracena, Dracena, SP, Brazil.
dc.description.affiliationUnespLaboratory of Metabolic and Toxicological Biochemistry (LaBMeT), UNESP - Univ Estadual Paulista, Campus of Dracena, Dracena, SP, Brazil. Electronic address: fmingatto@dracena.unesp.br.
dc.format.extent206-214
dc.identifierhttp://dx.doi.org/10.1016/j.etap.2015.06.013
dc.identifier.citationEnvironmental Toxicology And Pharmacology, v. 40, n. 1, p. 206-214, 2015.
dc.identifier.doi10.1016/j.etap.2015.06.013
dc.identifier.issn1872-7077
dc.identifier.pubmed26143514
dc.identifier.urihttp://hdl.handle.net/11449/131467
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofEnvironmental Toxicology And Pharmacology
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectFipronilen
dc.subjectLiveren
dc.subjectMetabolitesen
dc.subjectMitochondriaen
dc.subjectOxidative phosphorylationen
dc.subjectToxicityen
dc.titleComparative effects of fipronil and its metabolites sulfone and desulfinyl on the isolated rat liver mitochondriaen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Tecnológicas, Dracenapt

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