Cellulase production by trichosporon laibachii

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dc.contributor.authorGiese, Ellen C.
dc.contributor.authorDussán, Kelly J. [UNESP]
dc.contributor.authorPierozzi, Maurício
dc.contributor.authorChandel, Anuj K.
dc.contributor.authorPagnocca, Fernando C. [UNESP]
dc.contributor.authorDa Silva, Silvio S.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionCenter for Mineral Technology
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:15:11Z
dc.date.available2018-12-11T17:15:11Z
dc.date.issued2017-07-01
dc.description.abstractThe ability of twenty-three yeast strains isolated from decayed wood and the fungus garden of attine ants to produce hemi- and cellulolytic hydrolases in a chemically defined medium was acessed. Xylanase, β-glucosidase and cellulase activities were found in yeasts strains in the genera of Cryptococcus, Trichosporon, Debaryomyces and Pichia. Among the isolated microorganisms, two strains of Trichosporon laibachii MG270406-1A14 strain showed higher cellulase titers. Several biochemical and physiological parameters were investigated for optimum cellulase production under submerged fermentation. Enzyme induction was also examined using different carbon and nitrogen sources. Cellobiose and ammonium sulphate were found best carbon (C) and nitrogen (N) sources to enhance carboxymethylcellulase production. A 22- factorial design with center points was developed to optimize enzyme production, and data analysed by the response surface method. Maximal enzyme titres (0.3 U mL-1) occurred at initial pH 6.0, C/N ratio 9.0 and 5 days.en
dc.description.affiliationDepartment of Biotechnology School of Engineering of Lorena University of São Paulo
dc.description.affiliationCenter for Mineral Technology
dc.description.affiliationDepartment of Biochemistry and Chemical Technology Institute of Chemistry São Paulo State University-UNESP
dc.description.affiliationDepartment of Biochemistry and Microbiology Biosciences Institute of Rio Claro UNESP
dc.description.affiliationUnespDepartment of Biochemistry and Chemical Technology Institute of Chemistry São Paulo State University-UNESP
dc.description.affiliationUnespDepartment of Biochemistry and Microbiology Biosciences Institute of Rio Claro UNESP
dc.format.extent271-278
dc.identifierhttp://dx.doi.org/10.17807/orbital.v9i4.1024
dc.identifier.citationOrbital, v. 9, n. 4, p. 271-278, 2017.
dc.identifier.doi10.17807/orbital.v9i4.1024
dc.identifier.file2-s2.0-85030640412.pdf
dc.identifier.issn1984-6428
dc.identifier.scopus2-s2.0-85030640412
dc.identifier.urihttp://hdl.handle.net/11449/175294
dc.language.isoeng
dc.relation.ispartofOrbital
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCellulase
dc.subjectFermentation
dc.subjectTrichosporon
dc.subjectYeast
dc.titleCellulase production by trichosporon laibachiien
dc.typeArtigo
unesp.author.lattes8302605179522059[5]
unesp.author.orcid0000-0002-5026-1933[5]

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