Simple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensor

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dc.contributor.authorLamarca, Rafaela Silva [UNESP]
dc.contributor.authorFaria, Ricardo Adriano Dorledo De
dc.contributor.authorZanoni, Maria Valnice Boldrin [UNESP]
dc.contributor.authorNalin, Marcelo [UNESP]
dc.contributor.authorLima Gomes, Paulo Clairmont Feitosa De [UNESP]
dc.contributor.authorMessaddeq, Younès [UNESP]
dc.contributor.institutionUniversité Laval
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.date.accessioned2020-12-12T02:33:54Z
dc.date.available2020-12-12T02:33:54Z
dc.date.issued2020-01-01
dc.description.abstractIn this study an impedimetric immunosensor was developed in order to determine ciprofloxacin (CIP) in wastewater samples, an emergent contaminant widely found in wastewater. To achieve this, an anti-ciprofloxacin antibody was immobilized on the surface of a printed carbon electrode. Then, the developed immunosensor was applied in wastewater samples from Université Laval residences (Québec, Canada) through the load transfer resistance (Rct) using [Fe(CN)6]3-/4- as a redox probe, and the average CIP concentration was found to be 2.90 × 10-4 μg mL-1. The observed Rct changes presented a linear relationship from CIP concentrations of 10-5 to 1.0 μg mL-1, with detection and quantification limits of 2.50 × 10-6 and 7.90 × 10-6 μg mL-1, respectively. The immunosensor presented high selectivity and repeatability, as well as a good recovery rate in wastewater samples (97%). Significant interference with other compounds was not observed. The proposed method requires only 30 μL of sample without the use of organic solvents or preceding sample preparation and/or extraction techniques. Moreover, the method is fast: only 20 min of incubation followed by 2 min of analysis time was sufficient to obtain the CIP concentration. The method's estimated cost is U$ 2.00 per sample.en
dc.description.affiliationCenter for Optics Photonics and Laser (COPL) Université Laval
dc.description.affiliationUNESP Natl. Inst. for Alternative Technol. of Detection Toxicological Eval. and Removal of Micropollutants and Radioactives Institute of Chemistry São Paulo State University-UNESP, P.O. Box 355
dc.description.affiliationDepartment of Chemical Engineering Universidade Federal de Minas Gerais (UFMG)
dc.description.affiliationInstitute of Chemistry São Paulo State University (UNESP), P.O. Box 355
dc.description.affiliationUnespUNESP Natl. Inst. for Alternative Technol. of Detection Toxicological Eval. and Removal of Micropollutants and Radioactives Institute of Chemistry São Paulo State University-UNESP, P.O. Box 355
dc.description.affiliationUnespInstitute of Chemistry São Paulo State University (UNESP), P.O. Box 355
dc.format.extent1838-1847
dc.identifierhttp://dx.doi.org/10.1039/c9ra09083e
dc.identifier.citationRSC Advances, v. 10, n. 4, p. 1838-1847, 2020.
dc.identifier.doi10.1039/c9ra09083e
dc.identifier.issn2046-2069
dc.identifier.scopus2-s2.0-85078365384
dc.identifier.urihttp://hdl.handle.net/11449/201493
dc.language.isoeng
dc.relation.ispartofRSC Advances
dc.sourceScopus
dc.titleSimple, fast and environmentally friendly method to determine ciprofloxacin in wastewater samples based on an impedimetric immunosensoren
dc.typeArtigo

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