Crystal structure of human purine nucleoside phosphorylase at 2.3 angstrom resolution
dc.contributor.author | de Azevedo, W. F. | |
dc.contributor.author | Canduri, F. | |
dc.contributor.author | dos Santos, D. M. | |
dc.contributor.author | Silva, R. G. | |
dc.contributor.author | de Oliveira, J. S. | |
dc.contributor.author | de Carvalho, LPS | |
dc.contributor.author | Basso, L. A. | |
dc.contributor.author | Mendes, M. A. | |
dc.contributor.author | Palma, Mario Sergio [UNESP] | |
dc.contributor.author | Santos, D. S. | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Instituto Butantan | |
dc.contributor.institution | Universidade Federal do Rio Grande do Sul (UFRGS) | |
dc.contributor.institution | Pontificia Univ Catolica Rio Grande do Sul | |
dc.date.accessioned | 2014-05-20T13:54:37Z | |
dc.date.available | 2014-05-20T13:54:37Z | |
dc.date.issued | 2003-08-29 | |
dc.description.abstract | Purine nucleoside phosphorylase (PNP) catalyzes the phosphorolysis of the N-ribosidic bonds of purine nucleosides and deoxynucleosides. In human, PNP is the only route for degradation of deoxyguanosine and genetic deficiency of this enzyme leads to profound T-cell mediated immunosuppression. PNP is therefore a target for inhibitor development aiming at T-cell immune response modulation and its low resolution structure has been used for drug design. Here we report the structure of human PNP solved to 2.3 Angstrom resolution using synchrotron radiation and cryocrystallographic techniques. This structure allowed a more precise analysis of the active site, generating a more reliable model for substrate binding. The higher resolution data allowed the identification of water molecules in the active site, which suggests binding partners for potential ligands. Furthermore, the present structure may be used in the new structure-based design of PNP inhibitors. (C) 2003 Published by Elsevier B.V. | en |
dc.description.affiliation | UNESP, Dept Fis, BR-15054000 Sao Jose do Rio Preto, SP, Brazil | |
dc.description.affiliation | Inst Butantan, Ctr Appl Toxicol, BR-05503900 São Paulo, Brazil | |
dc.description.affiliation | UFRGS, Dept Mol Biol & Biotechnol, BR-91501970 Porto Alegre, RS, Brazil | |
dc.description.affiliation | UNESP, Inst Biosci, Dept Biol, Lab Struct Biol & Zoochem CEIS, BR-13506900 Rio Claro, SP, Brazil | |
dc.description.affiliation | Pontificia Univ Catolica Rio Grande do Sul, Fac Farm, Inst Pesquisas Biomed, Porto Alegre, RS, Brazil | |
dc.description.affiliationUnesp | UNESP, Dept Fis, BR-15054000 Sao Jose do Rio Preto, SP, Brazil | |
dc.description.affiliationUnesp | UNESP, Inst Biosci, Dept Biol, Lab Struct Biol & Zoochem CEIS, BR-13506900 Rio Claro, SP, Brazil | |
dc.format.extent | 545-552 | |
dc.identifier | http://dx.doi.org/10.1016/S0006-291X(03)01431-1 | |
dc.identifier.citation | Biochemical and Biophysical Research Communications. San Diego: Academic Press Inc. Elsevier B.V., v. 308, n. 3, p. 545-552, 2003. | |
dc.identifier.doi | 10.1016/S0006-291X(03)01431-1 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.lattes | 9424175688206545 | |
dc.identifier.lattes | 2901888624506535 | |
dc.identifier.uri | http://hdl.handle.net/11449/19546 | |
dc.identifier.wos | WOS:000184945400023 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | |
dc.relation.ispartofjcr | 2.559 | |
dc.rights.accessRights | Acesso restrito | |
dc.source | Web of Science | |
dc.subject | PNP | pt |
dc.subject | synchrotron radiation | pt |
dc.subject | Structure | pt |
dc.subject | drug design | pt |
dc.title | Crystal structure of human purine nucleoside phosphorylase at 2.3 angstrom resolution | en |
dc.type | Artigo | |
dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dcterms.rightsHolder | Elsevier B.V. | |
unesp.author.lattes | 9424175688206545 | |
unesp.author.lattes | 2901888624506535 | |
unesp.author.orcid | 0000-0002-2078-9286[8] | |
unesp.author.orcid | 0000-0002-7363-8211[9] | |
unesp.author.orcid | 0000-0003-0903-2407[7] | |
unesp.author.orcid | 0000-0003-4971-463X[10] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claro | pt |
unesp.campus | Universidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Preto | pt |
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