Dietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock protein

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dc.contributor.authorIshikawa, Larissa Lumi Watanabe [UNESP]
dc.contributor.authorFrança, Thaís Graziela Donegá [UNESP]
dc.contributor.authorChiuso-Minicucci, Fernanda [UNESP]
dc.contributor.authorZorzella-Pezavento, Sofia Fernanda Gonçalves [UNESP]
dc.contributor.authorMarra, Nelson Mendes [UNESP]
dc.contributor.authorPereira, Paulo Câmara Marques [UNESP]
dc.contributor.authorSilva, Célio Lopes
dc.contributor.authorSartori, Alexandrina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:23:56Z
dc.date.available2014-05-27T11:23:56Z
dc.date.issued2009-07-16
dc.description.abstractBackground: Protein-calorie malnutrition (PCM) is the most common type of malnutrition. PCM leads to immunodeficiency and consequent increased susceptibility to infectious agents. In addition, responses to prophylactic vaccines depend on nutritional status. This study aims to evaluate the ability of undernourished mice to mount an immune response to a genetic vaccine (pVAXhsp65) against tuberculosis, containing the gene coding for the heat shock protein 65 from mycobacteria. Methods: Young adult female BALB/c mice were fed ad libitum or with 80% of the amount of food consumed by a normal diet group. We initially characterized a mice model of dietary restriction by determining body and spleen weights, hematological parameters and histopathological changes in lymphoid organs. The ability of splenic cells to produce IFN-gamma and IL-4 upon in vitro stimulation with LPS or S. aureus and the serum titer of specific IgG1 and IgG2a anti-hsp65 antibodies after intramuscular immunization with pVAXhsp65 was then tested. Results: Dietary restriction significantly decreased body and spleen weights and also the total lymphocyte count in blood. This restriction also determined a striking atrophy in lymphoid organs as spleen, thymus and lymphoid tissue associated with the small intestine. Specific antibodies were not detected in mice submitted to dietary restriction whereas the well nourished animals produced significant levels of both, IgG1 and IgG2a anti-hsp65. Conclusion: 20% restriction in food intake deeply compromised humoral immunity induced by a genetic vaccine, alerting, therefore, for the relevance of the nutritional condition in vaccination programs based on these kinds of constructs. © 2009 Ishikawa et al; licensee BioMed Central Ltd.en
dc.description.affiliationDepartment of Microbiology and Immunology Biosciences Institute São Paulo State University (UNESP), Botucatu, São Paulo 18618-000
dc.description.affiliationDepartment of Parasitology Biosciences Institute São Paulo State University (UNESP), Botucatu, São Paulo 18618-000
dc.description.affiliationDepartment of Tropical Diseases Medical School São Paulo State University (UNESP), Botucatu, São Paulo 18618-000
dc.description.affiliationDepartment of Biochemistry and Immunology University of São Paulo (USP), Ribeirão Preto, São Paulo 14049-900
dc.description.affiliationUnespDepartment of Microbiology and Immunology Biosciences Institute São Paulo State University (UNESP), Botucatu, São Paulo 18618-000
dc.description.affiliationUnespDepartment of Parasitology Biosciences Institute São Paulo State University (UNESP), Botucatu, São Paulo 18618-000
dc.description.affiliationUnespDepartment of Tropical Diseases Medical School São Paulo State University (UNESP), Botucatu, São Paulo 18618-000
dc.identifierhttp://dx.doi.org/10.1186/1479-0556-7-11
dc.identifier.citationGenetic Vaccines and Therapy, v. 7.
dc.identifier.doi10.1186/1479-0556-7-11
dc.identifier.file2-s2.0-68449088131.pdf
dc.identifier.issn1479-0556
dc.identifier.lattes4977572416129527
dc.identifier.lattes1365320427418204
dc.identifier.orcid0000-0001-5771-8943
dc.identifier.scopus2-s2.0-68449088131
dc.identifier.urihttp://hdl.handle.net/11449/71090
dc.language.isoeng
dc.relation.ispartofGenetic Vaccines and Therapy
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDNA vaccine
dc.subjectgamma interferon
dc.subjectheat shock protein 65
dc.subjectimmunoglobulin G1
dc.subjectimmunoglobulin G2
dc.subjectinterleukin 4
dc.subjectanimal experiment
dc.subjectantibody production
dc.subjectatrophy
dc.subjectbody weight
dc.subjectcontrolled study
dc.subjectdiet restriction
dc.subjectfemale
dc.subjectfood intake
dc.subjectgenetic immunization
dc.subjecthematological parameters
dc.subjecthumoral immunity
dc.subjectimmune response
dc.subjectlymphocyte count
dc.subjectmalnutrition
dc.subjectmouse
dc.subjectMycobacterium tuberculosis
dc.subjectnonhuman
dc.subjectspleen cell
dc.subjectspleen weight
dc.subjectStaphylococcus aureus
dc.subjectAnimalia
dc.subjectCorynebacterineae
dc.subjectMus
dc.titleDietary restriction abrogates antibody production induced by a DNA vaccine encoding the mycobacterial 65 kDa heat shock proteinen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.lattes1365320427418204[6]
unesp.author.lattes4977572416129527[8]
unesp.author.orcid0000-0001-5771-8943[6]
unesp.author.orcid0000-0003-4557-3331[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB
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