Comparison Between Three Cryoprotectants in the Freezing ofMazama americanaSemen Collected by Artificial Vagina

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dc.contributor.authorAlvarez, Maria C. L. [UNESP]
dc.contributor.authorRola, Luciana D. [UNESP]
dc.contributor.authorDuarte, Jose M. B. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Fed Paraiba
dc.date.accessioned2020-12-10T20:04:52Z
dc.date.available2020-12-10T20:04:52Z
dc.date.issued2020-06-30
dc.description.abstractMaintaining genetic variability is an important part of the conservation of endangered species, so the construction of germplasm banks is essential. Several species of the genusMazamaendure constant pressure in their natural habitat and are threatened with extinction. The correct manipulation and adequacy of the diluents and cryoprotectants must be studied to be successful in the formation of these banks. The purpose of this study was to evaluate the efficiency of three different cryoprotectants in sperm cryopreservation in the speciesMazama americana: 6% glycerol (GLY), 3% ethylene glycol (ETG), and 5% dimethylformamide (DMF). Semen was obtained with the lateral deviation of the penis to an artificial vagina. In the pre-freeze and post-thaw periods, motility, vigor, membrane integrity, acrosome integrity, and sperm cell morphology were evaluated for each of the cryoprotectants. Post-thaw motility was higher when semen was frozen with cryoprotectants GLY and DMF (55.31 +/- 7.39 and 55.94 +/- 2.77, respectively), compared with the result obtained for ETG (48.13 +/- 2.39). For major defects (MaD), a difference was observed between the pre- and post-cryopreservation periods, such that DMF generated a higher number of post-thaw MaD (25.94 +/- 5.37). All cryoprotectants were efficient for cryopreservation ofM. americanasemen, resulting in samples with satisfactory viability after thawing. However, the medium with the cryoprotectants GLY, at a concentration of 6%, and DMF, at a concentration of 5%, were preferable.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, Nucleo Pesquisa & Conservacao Cervideos NUPECCE, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUniv Fed Paraiba, Dept Zootecnia, Ctr Ciencias Agr, Campus 2, Areia, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciencias Agr & Vet, Dept Zootecnia, Nucleo Pesquisa & Conservacao Cervideos NUPECCE, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/22962-9
dc.format.extent7
dc.identifierhttp://dx.doi.org/10.1089/bio.2020.0012
dc.identifier.citationBiopreservation And Biobanking. New Rochelle: Mary Ann Liebert, Inc, 7 p., 2020.
dc.identifier.doi10.1089/bio.2020.0012
dc.identifier.issn1947-5535
dc.identifier.urihttp://hdl.handle.net/11449/197060
dc.identifier.wosWOS:000547118600001
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc
dc.relation.ispartofBiopreservation And Biobanking
dc.sourceWeb of Science
dc.subjectgermplasm banks
dc.subjectdimethylformamide
dc.subjectethylene glycol
dc.subjectglycerol
dc.subjectdeer
dc.titleComparison Between Three Cryoprotectants in the Freezing ofMazama americanaSemen Collected by Artificial Vaginaen
dc.typeArtigo
dcterms.rightsHolderMary Ann Liebert, Inc

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