Occurrence of Cryptosporidium (Apicomplexa, Cryptosporidiidae) in Crotalus durissus terrificus (Serpentes, Viperidae) in Brazil

dc.contributor.authorKarasawa, Andréa Satie Matsubara [UNESP]
dc.contributor.authorSilva, Reinaldo José da [UNESP]
dc.contributor.authorMascarini, Luciene Maura [UNESP]
dc.contributor.authorBarrella, Thomaz Henrique [UNESP]
dc.contributor.authorLopes, Carlos Alberto de Magalhães [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:12:53Z
dc.date.available2014-05-20T13:12:53Z
dc.date.issued2002-09-01
dc.description.abstractThe objective of the present study was to investigate the prevalence of Cryptosporidium (Apicomplexa, Cryptosporidiidae) in the snake Crotalus durissus terrificus (Serpentes, Viperidae). Fifty animals were evaluated for the presence of oocysts of Cryptosporidium sp. at the time of arrival and 30 and 60 days later. Intestinal washings with saline solution (1% body weight), fecal samples, and organ scrapings were collected during the study. Oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by a density gradient centrifugation technique. Smears were made with the sediment and submitted to modified acid-fast and auramine-rhodamine staining. Cryptosporidium-positive smears were used as controls for the experimental findings. The overall prevalence of Cryptosporidium sp. oocysts was 14%. Among the positive snakes, oocysts were detected only in the intestinal washing in two specimens, only in the feces in four specimens, and in both materials at least once in one specimen. The positive snakes were predominantly from Santa Maria da Serra city State of São Paulo (57.1%). We also observed that all of the examinations that presented positive results were obtained at least 27 days after the capture of the animals.en
dc.description.affiliationUnesp Instituto de Biociências Departamento de Parasitologia
dc.description.affiliationUnesp Centro de Estudos de Venenos e Animais Peçonhentos
dc.description.affiliationUnespUnesp Instituto de Biociências Departamento de Parasitologia
dc.description.affiliationUnespUnesp Centro de Estudos de Venenos e Animais Peçonhentos
dc.format.extent779-781
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762002000600004
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 97, n. 6, p. 779-781, 2002.
dc.identifier.doi10.1590/S0074-02762002000600004
dc.identifier.fileS0074-02762002000600004.pdf
dc.identifier.issn0074-0276
dc.identifier.lattes5385706179871634
dc.identifier.scieloS0074-02762002000600004
dc.identifier.urihttp://hdl.handle.net/11449/792
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectCryptosporidiumen
dc.subjectCrotalus durissus terrificusen
dc.subjectsnakesen
dc.subjectBrazilen
dc.titleOccurrence of Cryptosporidium (Apicomplexa, Cryptosporidiidae) in Crotalus durissus terrificus (Serpentes, Viperidae) in Brazilen
dc.typeArtigo
unesp.author.lattes5385706179871634[3]

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