Cell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reaction

Página do item simplificado
dc.contributor.authorFecchio, C. J. [UNESP]
dc.contributor.authorDias-Melicio, L. A. [UNESP]
dc.contributor.authorSoares, Ângela Maria Victoriano de Campos [UNESP]
dc.contributor.authorGuimaraes, S. [UNESP]
dc.contributor.authorPeraçoli, Maria Terezinha Serrão [UNESP]
dc.contributor.authorSartori, Alexandrina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:37:19Z
dc.date.available2014-05-20T13:37:19Z
dc.date.issued2011-01-01
dc.description.abstractCell-free antigens (CFAg) derived from Paracoccidioides brasiliensis have typically been used in immunodiffusion reactions for serodiagnosis or therapeutic follow-up of paracoccidioidomycosis patients. Thus, we investigated the usefulness of CFAg obtained from cultures at different ages, to evaluate cellular immunity by the footpad test, in experimental murine paracoccidioidomycosis. Male mice infected with P. brasiliensis 265 strain were challenged in the footpad with CFAg obtained from four- (4d CFAg) or 11-day-old cultures (11d CFAg). The increase in footpad swelling provoked by 4d CFAg and 11d CFAg was similar and showed significant difference in relation to control groups. However, the infiltrate pattern was strikingly different: 4d CFAg induced a predominant mononuclear infiltrate whereas 11d CFAg provoked a predominant polymophonuclear infiltrate. These different inflammatory patterns were associated with distinct electrophoretic characteristics. By comparison with 11d CFAg, 4d CFAg showed more numerous and intense bands, including a strong one of 43 kDa (gp43). These results suggest that CFAg derived from Pb 265 isolate can be used as a reagent to evaluate cellular immunity; however, the culture's age is critical because only young cultures are able to induce a typical mononuclear infiltrate. The efficacy of this new paracoccidioidin to assay the cellular immunity in infections caused by other P. brasiliensis isolates is under investigation.en
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Microbiol & Immunol, Botucatu Biosci Inst, São Paulo State Univ, Botucatu, SP, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Postgrad Program Trop Dis, Botucatu Med Sch, São Paulo State Univ, Botucatu, SP, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Pathol, Botucatu Med Sch, São Paulo State Univ, Botucatu, SP, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Parasitol, Botucatu Biosci Inst, São Paulo State Univ, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Microbiol & Immunol, Botucatu Biosci Inst, São Paulo State Univ, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Postgrad Program Trop Dis, Botucatu Med Sch, São Paulo State Univ, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Pathol, Botucatu Med Sch, São Paulo State Univ, Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Dept Parasitol, Botucatu Biosci Inst, São Paulo State Univ, Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent318-324
dc.identifierhttp://dx.doi.org/10.1590/S1678-91992011000300012
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 17, n. 3, p. 318-324, 2011.
dc.identifier.fileS1678-91992011000300012-en.pdf
dc.identifier.issn1678-9199
dc.identifier.lattes4977572416129527
dc.identifier.lattes6486557387397806
dc.identifier.scieloS1678-91992011000300012
dc.identifier.urihttp://hdl.handle.net/11449/12894
dc.identifier.wosWOS:000294438900011
dc.language.isoeng
dc.publisherUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.relation.ispartofjcr1.782
dc.relation.ispartofsjr0,573
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectParacoccidioides brasiliensisen
dc.subjectcell-free antigensen
dc.subjectdelayed-type hypersensitivity reactionen
dc.subjectgp43en
dc.subjectmurine paracoccidioidomycosisen
dc.titleCell-free antigens from precocious Paracoccidioides brasiliensis culture induce a typical delayed-type hypersensitivity reactionen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jvatitd/iaboutj.htm
dcterms.rightsHolderCevap-unesp
unesp.advisor.lattes6486557387397806
unesp.author.lattes4977572416129527[6]
unesp.author.orcid0000-0003-4557-3331[6]
unesp.author.orcid0000-0003-1962-9901[3]
unesp.author.orcid0000-0001-9254-2074[2]
unesp.author.orcid0000-0003-3704-8131[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S1678-91992011000300012-en.pdf
Tamanho:
335.08 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Microbiologia e Imunologia - IBB
Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB
Artigos - Parasitologia - IBB
Artigos - Patologia - FMB