Prototheca blaschkeae subsp. Brasiliensis subsp. nov., isolated from cow milk

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dc.contributor.authorMorandi, Stefano
dc.contributor.authorCremonesi, Paola
dc.contributor.authorPovolo, Milena
dc.contributor.authorCapra, Emanuele
dc.contributor.authorSilvetti, Tiziana
dc.contributor.authorCastiglioni, Bianca
dc.contributor.authorRibeiro, Márcio Garcia [UNESP]
dc.contributor.authorAlves, Ana Carolina [UNESP]
dc.contributor.authorda Costa, Geraldo Márcio
dc.contributor.authorLuini, Mario
dc.contributor.authorBrasca, Milena
dc.contributor.institutionItalian National Research Council (CNR ISPA)
dc.contributor.institutionItalian National Research Council (CNR IBBA)
dc.contributor.institutionCouncil for Agricultural Research and Economics – Research Centre for Animal Production and Aquaculture (CREA-ZA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionFederal University of Lavras
dc.contributor.institutionIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER)
dc.date.accessioned2018-12-11T17:34:33Z
dc.date.available2018-12-11T17:34:33Z
dc.date.issued2017-10-01
dc.description.abstractA strain of an achlorophyllic alga, named PR24T, was isolated from cow milk samples from the state of Minas Gerais, Brazil. Based on 18S rDNA, 28S rRNA, D1/D2 region of the LSU rDNA and SSU rRNA gene sequence similarities, this strain was found to be a member of the genus Prototheca and closely related to Prototheca blaschkeae SAG2064T. However, the novel strain could easily be distinguished from recognized Prototheca species by internal transcribed spacer, species-specific PCR, single-strand conformation polymorphism-PCR analysis and phenotypic characteristics. The inability to grow in Sabouraud broth at pH 4.0 and the different cellular fatty acid composition clearly distinguished PR24T from the reference strain of P. blaschkeae. The combination of genotypic and phenotypic data indicates that strain PR24T represents a subspecies of P. blaschkeae, for which the name Prototheca blaschkeae subsp. brasiliensis subsp. nov. is proposed. The respective type strain is PR24T (=DSM 103592T=IHEM 26958T).en
dc.description.affiliationInstitute of Sciences of Food Production Italian National Research Council (CNR ISPA), Via Celoria 2
dc.description.affiliationInstitute of Agricultural Biology and Biotechnology Italian National Research Council (CNR IBBA), Via Einstein
dc.description.affiliationCouncil for Agricultural Research and Economics – Research Centre for Animal Production and Aquaculture (CREA-ZA), Via Lombardo 11
dc.description.affiliationDepartment of Veterinary Hygiene and Public Health Universidade Estadual Paulista (UNESP)
dc.description.affiliationDepartment of Veterinary Medicine Federal University of Lavras
dc.description.affiliationIstituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Via Einstein
dc.description.affiliationUnespDepartment of Veterinary Hygiene and Public Health Universidade Estadual Paulista (UNESP)
dc.format.extent3865-3871
dc.identifierhttp://dx.doi.org/10.1099/ijsem.0.002209
dc.identifier.citationInternational Journal of Systematic and Evolutionary Microbiology, v. 67, n. 10, p. 3865-3871, 2017.
dc.identifier.doi10.1099/ijsem.0.002209
dc.identifier.file2-s2.0-85031809611.pdf
dc.identifier.issn1466-5026
dc.identifier.scopus2-s2.0-85031809611
dc.identifier.urihttp://hdl.handle.net/11449/179285
dc.language.isoeng
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiology
dc.relation.ispartofsjr0,943
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCow milk
dc.subjectPrototheca blaschkeae subsp. brasiliensis
dc.subjectProtothecal bovine mastitis
dc.titlePrototheca blaschkeae subsp. Brasiliensis subsp. nov., isolated from cow milken
dc.typeArtigo

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