Improving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distribution

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dc.contributor.authorPerez, Caroline Lopes [UNESP]
dc.contributor.authorCasciatori, Fernanda Perpétua
dc.contributor.authorThoméo, João Cláudio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.date.accessioned2021-06-25T11:07:27Z
dc.date.available2021-06-25T11:07:27Z
dc.date.issued2021-03-01
dc.description.abstractEnzymes production by solid-state cultivation in packed-bed bioreactor needs to be improved by mathematical modeling and also by experimentation. In this work, a mixture of sugarcane bagasse and wheat bran was used for the growth of the fungus Myceliophthora thermophila I-1D3b, able to secrete endoglucanase and xylanase, enzymes of interest in the second-generation ethanol production. Bench and pilot-scale bioreactors were used for the experiments, while critical parameters as bed porosity and airflow distribution were evaluated. Results showed enzymes with higher activities for the most porous medium, even though the less substrate amount to be cultivated. For the pilot-scale bioreactor, only the most porous medium was evaluated using different airflow distribution techniques. Using an inner tube for air supply resulted in more homogeneous enzyme production, with higher activities. The results here presented will be helpful for the scale-up of this class of bioreactor into industrial apparatuses.en
dc.description.affiliationFood Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo, 2265, Jardim Nazareth
dc.description.affiliationGraduate Program of Chemical Engineering Federal University of São Carlos (UFSCar), Rod. Washington Luiz km 235, SP-310
dc.description.affiliationUnespFood Engineering and Technology Department Institute of Biosciences Letters and Exact Sciences São Paulo State University (UNESP), Cristóvão Colombo, 2265, Jardim Nazareth
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 132957/2016-7
dc.description.sponsorshipIdFAPESP: 2014/23453-3
dc.description.sponsorshipIdFAPESP: 2018/00996-2
dc.description.sponsorshipIdCNPq: 430786/2018-2
dc.format.extent537-548
dc.identifierhttp://dx.doi.org/10.1007/s00449-020-02466-7
dc.identifier.citationBioprocess and Biosystems Engineering, v. 44, n. 3, p. 537-548, 2021.
dc.identifier.doi10.1007/s00449-020-02466-7
dc.identifier.issn1615-7605
dc.identifier.issn1615-7591
dc.identifier.scopus2-s2.0-85096397336
dc.identifier.urihttp://hdl.handle.net/11449/208164
dc.language.isoeng
dc.relation.ispartofBioprocess and Biosystems Engineering
dc.sourceScopus
dc.subjectCellulase
dc.subjectFixed bioreactor
dc.subjectPacking density
dc.subjectScale-up
dc.subjectSolid-state fermentation
dc.titleImproving enzyme production by solid-state cultivation in packed-bed bioreactors by changing bed porosity and airflow distributionen
dc.typeArtigo

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Artigos - Engenharia e Tecnologia de Alimentos - IBILCE