Cariotipo de la especie invasora Pterois volitans (Scorpaeniformes: Scorpaenidae) de Isla Margarita Island, Venezuela

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dc.contributor.authorNirchio, Mauro
dc.contributor.authorEhemann, Nicolás
dc.contributor.authorSiccha-Ramirez, Raquel [UNESP]
dc.contributor.authorRon, Ernesto
dc.contributor.authorEduardo Pérez, Julio
dc.contributor.authorRita Rossi, Anna
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.institutionUniversidad de Oriente
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Rome ‘La Sapienza’
dc.date.accessioned2022-04-28T19:00:39Z
dc.date.available2022-04-28T19:00:39Z
dc.date.issued2014-01-01
dc.description.abstractThe genus Pterois includes nine valid species, native to the Red Sea and Indian Ocean throughout the Western Pacific. P. volitans and P. miles are native to the Indo-Pacific, and were introduced into Florida waters as a result of aquarium releases, and have been recently recognized as invaders of the Western Atlantic and Caribbean Sea (Costa Rica to Venezuela). Thus far, cytogenetic studies of the genus Pterois only cover basic aspects of three species, including P. volitans from Indo-Pacific Ocean. Considering the lack of more detailed information about cytogenetic characteristics of this invasive species, the objective of the present study was to investigate the basic and molecular cytogenetic characteristics of P. volitans in Venezuela, and compare the results with those from the original distribution area. For this, the karyotypic characteristics of four lionfish caught in Margarita Island, Venezuela, were investigated by examining metaphase chromosomes by Giemsa staining, C-banding, Ag-NOR, and two-colour-Fluorescent in situ hybridization (FISH) for mapping of 18S and 5S ribosomal genes. Comparing the sequences of the 16S gene of the specimens analyzed, with sequences already included in the Genbank, we corroborated that our specimens identified as P. volitans are in fact this species, and hence exclude the possibility of a misidentification of P. miles. The diploid number was 2n=48 (2m+10sm+36a) with FN=60. Chromosomes uniformly decreased in size, making it difficult to clearly identify the homologues except for the only metacentric pair, and the pairs number two, the largest of the submetacentric series. C-banding revealed only three pairs of chromosomes negative for C-band, whereas all remaining chromosomes presented telomeric and some interstitial C-positive blocks. Only two chromosomes were C-banding positive at the pericentromeric regions. Sequential staining revealed Ag-NOR on the tips of the short arms of chromosome pair number two and the FISH assay revealed that 18S rDNA and 5S rDNA genes are co-located on this chromosome pair. The co-localization of 5S rDNA and 45S rDNA is discussed. Both constitutive heterochromatin and NOR location detected in samples examined in this study, differ from those reported for P. volitans in previous analysis of specimens collected in Indian Ocean (Java), suggesting the occurrence of chromosome microrearrangements involving heterochromatin during the spread of P. volitans.en
dc.description.affiliationEscuela de Ciencias Aplicadas del Mar Universidad de Oriente
dc.description.affiliationDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista
dc.description.affiliationInstituto Oceanográfico de Venezuela Universidad de Oriente
dc.description.affiliationDepartment of Biology and Biotechnology University of Rome ‘La Sapienza’, via Borelli 50
dc.description.affiliationUnespDepartamento de Morfologia Instituto de Biociências Universidade Estadual Paulista
dc.format.extent1365-1374
dc.identifierhttp://dx.doi.org/10.15517/rbt.v62i4.13029
dc.identifier.citationRevista de Biologia Tropical, v. 62, n. 4, p. 1365-1374, 2014.
dc.identifier.doi10.15517/rbt.v62i4.13029
dc.identifier.issn2215-2075
dc.identifier.issn0034-7744
dc.identifier.scopus2-s2.0-84921930004
dc.identifier.urihttp://hdl.handle.net/11449/220297
dc.language.isoeng
dc.relation.ispartofRevista de Biologia Tropical
dc.sourceScopus
dc.subject16S rRNA mitochondrial gene
dc.subject18S and 5S ribosomal genes
dc.subjectInvasive species
dc.subjectKaryotype
dc.subjectMolecular identification
dc.titleCariotipo de la especie invasora Pterois volitans (Scorpaeniformes: Scorpaenidae) de Isla Margarita Island, Venezuelaes
dc.title.alternativeKaryotype of the invasive species Pterois volitans (Scorpaeniformes: Scorpaenidae) from Margarita Island, Venezuelaen
dc.typeArtigo

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