Cryopreservation of boar semen in 0.5mL straws at low spermatozoa concentration is better than high concentration to maintain sperm viability

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dc.contributor.authorRavagnani, Gisele M.
dc.contributor.authorTorres, Mariana A.
dc.contributor.authorLeal, Diego F.
dc.contributor.authorMartins, Simone M. M. K.
dc.contributor.authorPapa, Frederico O. [UNESP]
dc.contributor.authorDell'Aqua Junior, Jose A. [UNESP]
dc.contributor.authorAlvarenga, Marco A. [UNESP]
dc.contributor.authorAndrade, Andre F. C.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2019-10-04T12:32:09Z
dc.date.available2019-10-04T12:32:09Z
dc.date.issued2018-09-01
dc.description.abstractTo date, no studies have been performed evaluating the effect of boar spermatozoa concentration in 0.5mL freezing straws, leading us to examine this question. Each sperm-rich fraction of the ejaculate (n=25) was diluted at five different sperm concentrations (100, 200, 300, 600 and 800 x 10(6) spermatozoa/mL), packaged in 0.5mL straws, and subsequently frozen. After thawing, the sperm from all of treatment groups were analyzed to determine motility characteristics using a sperm class analyzer (SCA-CASA), and their plasma and acrosomal membrane integrity, mitochondria! membrane potential, sperm membrane lipid peroxidation and fluidity were analyzed by flow cytometry. An increase in spermatozoa concentration above 300x10(6) spermatozoa/mL in a 0.5mL straw impaired (p<0.05) the total and progressive motility, curvilinear velocity, straight-line velocity, linearity and beat cross frequency. However, the plasma and acrosomal membrane integrity, mitochondria] membrane potential, membrane lipid peroxidation and fluidity were not influenced (p>0.05) by high spermatozoa concentrations at freezing. Therefore, to increase spermatozoa survival and total and progressive motility after thawing, boar spermatozoa should be frozen at concentrations up to 300x10(6) spermatozoa/mL.en
dc.description.affiliationUniv Sao Paulo, Fac Med Vet & Zootecnia, Nucl Pesquisa Suinos, BR-13635900 Pirassununga, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, UNESP, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Med Vet & Zootecnia, Dept Reprod Anim & Radiol Vet, UNESP, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/23484-8
dc.format.extent1726-1730
dc.identifierhttp://dx.doi.org/10.1590/1678-5150-PVB-5465
dc.identifier.citationPesquisa Veterinaria Brasileira. Rio Janeiro: Revista Pesquisa Veterinaria Brasileira, v. 39, n. 9, p. 1726-1730, 2018.
dc.identifier.doi10.1590/1678-5150-PVB-5465
dc.identifier.fileS0100-736X2018000901726.pdf
dc.identifier.issn0100-736X
dc.identifier.scieloS0100-736X2018000901726
dc.identifier.urihttp://hdl.handle.net/11449/185034
dc.identifier.wosWOS:000449559300003
dc.language.isoeng
dc.publisherRevista Pesquisa Veterinaria Brasileira
dc.relation.ispartofPesquisa Veterinaria Brasileira
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCryopreservation
dc.subjectboar semen
dc.subjectspermatozoa concentration
dc.subjectsperm viability
dc.subjectcryoinjury
dc.subjectcryocapacitation
dc.subjectswine
dc.titleCryopreservation of boar semen in 0.5mL straws at low spermatozoa concentration is better than high concentration to maintain sperm viabilityen
dc.typeArtigo
dcterms.rightsHolderRevista Pesquisa Veterinaria Brasileira

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