Acrosomal ultrastructure of stallion spermatozoa cryopreserved with ethylene glycol using two packaging systems

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dc.contributor.authorAlvarenga, Marco Antonio [UNESP]
dc.contributor.authorLandim-Alvarenga, F. C.
dc.contributor.authorMoreira, R. M.
dc.contributor.authorCesarino, M. M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-02-26T17:10:50Z
dc.date.accessioned2014-05-20T13:41:40Z
dc.date.available2014-02-26T17:10:50Z
dc.date.available2014-05-20T13:41:40Z
dc.date.issued2000-11-01
dc.description.abstractThe present experiments aimed to examine the substitution of glycerol (G) by ethylene glycol (E) as a cryoprotective agent for stallion spermatozoa. Two different ethylene glycol concentrations (5% and 10%) and also the association of glycerol (2%) and ethylene glycol (3%) (E/G) were studied (Experiment 1). In Experiment 2, two packing systems (0.5 x 4.0 ml) were evaluated using both cryoprotectors. In both experiments, the sperm membrane integrity after freezing was evaluated using transmission electron microscopy. The mean post-thaw motility was 34.25, 36.5, 29.25 and 34.75% for G5%, E5%, E10% and E/G, respectively. It was observed that the percentage of motile spermatozoa was significantly smaller (P<0.05) when semen was processed with E10%. A decrease in the acrosome integrity was observed in frozen thawed spermatozoa from all treated groups. It was observed that 28.0, 22.5, 25.5 and 22.5 % of the sperm cells had a normal acrosome following freezing with G5%, E5%, E10% and E/G, respectively. Undulation of the outer acrosomal membrane, acrosomal swelling and loss of acrosomal content density and homogeneity were the most evident ultrastructural alterations observed. In Experiment 2, the post-thaw motility was higher (P<0.05) for sperm frozen in 0.5 ml straws than in 4.0 mi straws, regardless of the cryoprotector used. The ultrastructural evaluation showed 26.7 and 16.0% of intact acrosomes for sperm frozen in 0.5 ml and 4.0 ml straws, respectively. We concluded that ethylene glycol has similar cryoprotective properties to glycerol and that utilisation of 0.5 ml straws improved the ability of horse sperm cells to withstand damage after the cryopreservation process.en
dc.description.affiliationUNESP, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, BR-18618 Botucatu, SP, Brazil
dc.description.affiliationUnespUNESP, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, BR-18618 Botucatu, SP, Brazil
dc.format.extent541-545
dc.identifierhttp://dx.doi.org/10.2746/042516400777584749
dc.identifier.citationEquine Veterinary Journal. Newmarket: Equine Veterinary Journal Ltd, v. 32, n. 6, p. 541-545, 2000.
dc.identifier.doi10.2746/042516400777584749
dc.identifier.issn0425-1644
dc.identifier.lattes0473846154288947
dc.identifier.lattes8456490300814833
dc.identifier.orcid0000-0002-2420-2550
dc.identifier.urihttp://hdl.handle.net/11449/14439
dc.identifier.wosWOS:000165445900013
dc.language.isoeng
dc.publisherEquine Veterinary Journal Ltd
dc.relation.ispartofEquine Veterinary Journal
dc.relation.ispartofjcr2.022
dc.relation.ispartofsjr0,991
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjecthorsept
dc.subjectcryoprotectorpt
dc.subjectstallionpt
dc.subjectsemenpt
dc.subjectethylene glycolpt
dc.subjectultrastructurept
dc.titleAcrosomal ultrastructure of stallion spermatozoa cryopreserved with ethylene glycol using two packaging systemsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderEquine Veterinary Journal Ltd
unesp.author.lattes0473846154288947
unesp.author.lattes8456490300814833[2]
unesp.author.orcid0000-0002-2420-2550[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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