Investigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potential

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dc.contributor.authorCardoso, Fl�via Goulart Da Rosa [UNESP]
dc.contributor.authorChung, Adriana [UNESP]
dc.contributor.authorMartinho, Frederico Canato [UNESP]
dc.contributor.authorCamargo, Carlos Henrique Ribeiro [UNESP]
dc.contributor.authorCarvalho, Claudio Ant�nio Talge [UNESP]
dc.contributor.authorGomes, Brenda Paula Figueiredo De Almeida
dc.contributor.authorValera, Marcia Carneiro [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUNITAU - Universidade de Taubat�
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2018-12-11T17:29:38Z
dc.date.available2018-12-11T17:29:38Z
dc.date.issued2016-07-01
dc.description.abstractThis clinical study investigated and quantified cultivable bacteria and their levels of endotoxins in persistent endodontic infection, determining their antigenicity against macrophages and fibroblast cells by IL-1β and TNF-α secretion and evaluating their relationship with clinical and radiographic features. Samples from the root canals were obtained after root filling removal. Culture techniques were used to determine the bacterial count and the endotoxins were determined by LAL-assay. PCR analysis (16S rDNA) was used for bacterial detection. Raw 264.5 macrophages and V79 fibroblast were stimulated with endodontic contents. ELISA assay measured the amounts of IL-1�/TNF- □secretion. Bacteria and endotoxin medians were 1.24x105 CFU/mL and 9.62 EU/mL, respectively. Porphyromonas endodontalis was the most frequently detected species. Higher levels of endotoxins were found in teeth with pain on palpation (23.56 EU/mL) rather than in its absence (8.21 EU/mL). Larger areas of bone destruction were related to higher levels of endotoxins and IL-1β and TNF-α secretion. The study findings revealed the presence of Gram-negative bacteria species in persistent endodontic infection, with their endotoxins related to both severity of bone destruction and development of symptomatology. Moreover, larger areas of bone destruction were related to higher levels of IL-1β and TNF-α secreted by macrophages and fibroblast cells.en
dc.description.affiliationDepartment of Restorative Dentistry Endodontic Division Institute of Science and Technology UNESP – Univ Estadual Paulista
dc.description.affiliationDepartment of Odontology Endodontic Division UNITAU - Universidade de Taubat�
dc.description.affiliationDepartment of Restorative Dentistry Endodontic Division Piracicaba Dental School UNICAMP - Universidade Estadual de Campinas
dc.description.affiliationUnespDepartment of Restorative Dentistry Endodontic Division Institute of Science and Technology UNESP – Univ Estadual Paulista
dc.format.extent412-418
dc.identifierhttp://dx.doi.org/10.1590/0103-6440201600520
dc.identifier.citationBrazilian Dental Journal, v. 27, n. 4, p. 412-418, 2016.
dc.identifier.doi10.1590/0103-6440201600520
dc.identifier.fileS0103-64402016000400412.pdf
dc.identifier.issn1806-4760
dc.identifier.issn0103-6440
dc.identifier.scieloS0103-64402016000400412
dc.identifier.scopus2-s2.0-84988433786
dc.identifier.urihttp://hdl.handle.net/11449/178286
dc.language.isoeng
dc.relation.ispartofBrazilian Dental Journal
dc.relation.ispartofsjr0,476
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBacteria
dc.subjectCytokines
dc.subjectEndodontic infection
dc.subjectEndotoxin
dc.subjectRoot canals
dc.titleInvestigation of bacterial contents from persistent endodontic infection and evaluation of their inflammatory potentialen
dc.typeArtigo

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