Diffusion ability of endotoxin through dentinal tubules

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dc.contributor.authorOliveira, Luciane Dias de
dc.contributor.authorCarvalho, Cláudio Antonio Talge
dc.contributor.authorValera, Marcia Carneiro
dc.contributor.authorKoga-Ito, Cristiane Yumi
dc.contributor.authorJorge, Antonio Olavo Cardoso [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:21:15Z
dc.date.available2014-05-27T11:21:15Z
dc.date.issued2005-01-01
dc.description.abstractThe aim of this study was to evaluate the ability of endotoxin to diffuse through dentinal tubules towards the cement and to observe the period of time needed for it to reach the external root surface. Thirty single-rooted human teeth had their crowns and apices removed in order to standardize the root length to 15 mm. Teeth were instrumented until #30 K-file and made externally impermeable with epoxy adhesive, leaving 10 mm of the exposed root (middle third). The specimens were placed in plastic vials and irradiated (60Co gamma-rays). Then, they were divided into 2 groups (n = 15): G1) Escherichia coli endotoxin was inoculated into the root canal of the specimens and 1 ml of pyrogen-free water was put in the tubes; G2) (control): pyrogen-free water was inoculated into the root canals and 1 ml of pyrogen-free water was put in each tube. After 30 min, 2 h, 6 h, 12 h, 24 h, 48 h, 72 h and 7 days, the water of the tubes was removed and replaced. The removed aliquot was tested for the presence of endotoxin. Considering that the endotoxin is a B-lymphocyte polyclonal activator, at each experimental period, B-lymphocyte culture was stimulated with a sample of water removed from each tube and antibody (IgM) production was detected by ELISA technique. The results of IgM production were higher in groups of 24 h, 48 h, 72 h and 7 days in relation to the other studied groups, with statistically significant differences (ANOVA and Tukey's test p < 0.05). Endotoxin was able to diffuse through the dentinal tubules towards the cement, reaching the external root surface after the period of 24 h.en
dc.format.extent5-10
dc.identifierhttp://dx.doi.org/10.1590/S1806-83242005000100002
dc.identifier.citationPesquisa odontológica brasileira = Brazilian oral research., v. 19, n. 1, p. 5-10, 2005.
dc.identifier.doi10.1590/S1806-83242005000100002
dc.identifier.file2-s2.0-33645897049.pdf
dc.identifier.issn1806-8324
dc.identifier.lattes4716676611330311
dc.identifier.lattes0053567153623569
dc.identifier.lattes9304600166583100
dc.identifier.lattes6543563161403421
dc.identifier.orcid0000-0003-0987-5594
dc.identifier.orcid0000-0002-2416-2173
dc.identifier.scieloS1806-83242005000100002
dc.identifier.scopus2-s2.0-33645897049
dc.identifier.urihttp://hdl.handle.net/11449/68096
dc.language.isoeng
dc.relation.ispartofPesquisa Odontológica Brasileira = Brazilian Oral Research
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectendotoxin
dc.subjectendotoxin, Escherichia coli
dc.subjectimmunoglobulin G
dc.subjecttooth cement
dc.subjectanalysis of variance
dc.subjectanimal
dc.subjectB lymphocyte
dc.subjectchemistry
dc.subjectculture medium
dc.subjectdentin
dc.subjectdiffusion
dc.subjectenzyme linked immunosorbent assay
dc.subjecthuman
dc.subjectimmunology
dc.subjectmale
dc.subjectmetabolism
dc.subjectmicrobiology
dc.subjectmouse
dc.subjecttime
dc.subjecttooth permeability
dc.subjecttooth pulp
dc.subjectAnalysis of Variance
dc.subjectAnimals
dc.subjectB-Lymphocytes
dc.subjectCulture Media
dc.subjectDental Cements
dc.subjectDental Pulp Cavity
dc.subjectDentin
dc.subjectDentin Permeability
dc.subjectDiffusion
dc.subjectEndotoxins
dc.subjectEnzyme-Linked Immunosorbent Assay
dc.subjectHumans
dc.subjectImmunoglobulin G
dc.subjectMale
dc.subjectMice
dc.subjectTime Factors
dc.titleDiffusion ability of endotoxin through dentinal tubulesen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/pob/paboutj.htm#Propriedade
unesp.author.lattes4716676611330311
unesp.author.lattes0053567153623569[5]
unesp.author.lattes9304600166583100[2]
unesp.author.lattes6543563161403421[4]
unesp.author.orcid0000-0001-7398-6438[3]
unesp.author.orcid0000-0002-1747-6158[5]
unesp.author.orcid0000-0003-0987-5594[2]
unesp.author.orcid0000-0002-2416-2173[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Ciência e Tecnologia, São José dos Campospt

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