Distribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifrices

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dc.contributor.authorPessan, Juliano Pelim [UNESP]
dc.contributor.authorRibeiro Pinto Alves, Karina Mirela [UNESP]
dc.contributor.authorItaliani, Flavia de Moraes
dc.contributor.authorRamires, Irene
dc.contributor.authorPereira Lauris, Jose Roberto
dc.contributor.authorWhitford, Gary Milton
dc.contributor.authorToumba, Kyriacos Jack
dc.contributor.authorRobinson, Colin
dc.contributor.authorRabelo Buzalaf, Marilia Afonso
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionGeorgia Regents Univ
dc.contributor.institutionUniv Leeds
dc.date.accessioned2014-12-03T13:10:44Z
dc.date.available2014-12-03T13:10:44Z
dc.date.issued2014-07-01
dc.description.abstractBackground. The distribution of fluoride and calcium in plaque after the use of fluoride dentifrices has not yet been determined.Aim. To evaluate fluoride and calcium distribution in sections of biofilms generated in situ after the use of conventional and low-fluoride dentifrices.Design. Children (n = 11, 8-10 years old) brushed with placebo (fluoride-free), low-fluoride (513 mgF/kg), and conventional (1072 mgF/kg) dentifrices twice daily for 1 week, following a double-blind, cross-over protocol. Biofilms were generated using Leeds in situ devices, which were collected 1 and 12 h after brushing, and sectioned through their depth. Sections were grouped (10 x 5 mu m) for fluoride and calcium analysis. Sections 4 mu m thick were used for image analysis and determination of biomass fraction. Results were analysed by ANOVA, Tukey's test, and linear regression analysis (P < 0.05).Results. Fluoride and calcium were mostly located at the outer sections of biofilms for all dentifrices tested, and these ions were directly correlated throughout most of biofilm's sections. Results for conventional dentifrice were significantly higher than for the placebo, but did not differ from those for the low-fluoride dentifrice.Conclusions. The use of a low-fluoride dentifrice did not promote a higher fluoride uptake in inner biofilms' sections, as hypothesized. As plaque fluoride was significantly elevated only after the use of the conventional dentifrice, the recommendation of low-fluoride formulations should be done with caution, considering both risks and benefits.en
dc.description.affiliationUniv Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, BR-16015050 Aracatuba, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Bauru Dent Sch, Dept Biol Sci, Bauru, Brazil
dc.description.affiliationUniv Sao Paulo, Bauru Dent Sch, Dept Pediat Dent Orthodont & Publ Hlth, Bauru, Brazil
dc.description.affiliationGeorgia Regents Univ, Dept Physiol, Augusta, GA USA
dc.description.affiliationUniv Leeds, Leeds Dent Inst, Div Child Dent Hlth, Leeds, W Yorkshire, England
dc.description.affiliationUniv Leeds, Div Oral Biol, Leeds Dent Inst, Leeds, W Yorkshire, England
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Aracatuba Dent Sch, Dept Pediat Dent & Publ Hlth, BR-16015050 Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent293-302
dc.identifierhttp://dx.doi.org/10.1111/ipd.12073
dc.identifier.citationInternational Journal Of Paediatric Dentistry. Hoboken: Wiley-blackwell, v. 24, n. 4, p. 293-302, 2014.
dc.identifier.doi10.1111/ipd.12073
dc.identifier.issn0960-7439
dc.identifier.urihttp://hdl.handle.net/11449/112460
dc.identifier.wosWOS:000337699400006
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofInternational Journal of Paediatric Dentistry
dc.relation.ispartofjcr1.383
dc.relation.ispartofsjr0,699
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleDistribution of fluoride and calcium in plaque biofilms after the use of conventional and low-fluoride dentifricesen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.orcid0000-0003-3475-4479[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt

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