Expression, purification and molecular analysis of the human ZNF706 protein

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dc.contributor.authorColombo, Jucimara [UNESP]
dc.contributor.authorProvazzi, Paola Jocelan Scarin [UNESP]
dc.contributor.authorCalmon, Marilia Freitas [UNESP]
dc.contributor.authorPires, Lilian Campos [UNESP]
dc.contributor.authorRodrigues, Nathália Campos
dc.contributor.authorPetl, Paulo [UNESP]
dc.contributor.authorFossey, Marcelo Andrés [UNESP]
dc.contributor.authorDe Souza, Fátima Pereira [UNESP]
dc.contributor.authorCanduri, Fernanda
dc.contributor.authorRahal, Paula [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:30:45Z
dc.date.available2014-05-27T11:30:45Z
dc.date.issued2013-09-25
dc.description.abstractBackground: The ZNF706 gene encodes a protein that belongs to the zinc finger family of proteins and was found to be highly expressed in laryngeal cancer, making the structure and function of ZNF706 worthy of investigation. In this study, we expressed and purified recombinant human ZNF706 that was suitable for structural analysis in Escherichia coli BL21(DH3). Findings. ZNF706 mRNA was extracted from a larynx tissue sample, and cDNA was ligated into a cloning vector using the TOPO method. ZNF706 protein was expressed according to the E. coli expression system procedures and was purified using a nickel-affinity column. The structural qualities of recombinant ZNF706 and quantification alpha, beta sheet, and other structures were obtained by spectroscopy of circular dichroism. ZNF706's structural modeling showed that it is composed of α-helices (28.3%), β-strands (19.4%), and turns (20.9%), in agreement with the spectral data from the dichroism analysis. Conclusions: We used circular dichroism and molecular modeling to examine the structure of ZNF706. The results suggest that human recombinant ZNF706 keeps its secondary structures and is appropriate for functional and structural studies. The method of expressing ZNF706 protein used in this study can be used to direct various functional and structural studies that will contribute to the understanding of its function as well as its relationship with other biological molecules and its putative role in carcinogenesis. © 2013 Colombo et al.; licensee BioMed Central Ltd.en
dc.description.affiliationDepartment of Biology São Paulo State University UNESP, CEP: 15054-000, São José do Rio Preto /SP
dc.description.affiliationInstitute of Chemistry of São Carlos Department of Chemistry and Molecular Physics University of São Paulo - USP, CEP: 13560-970, São Carlos /SP
dc.description.affiliationDepartment of Physics São Paulo State University UNESP, CEP: 15054-000, São José do Rio Preto /SP
dc.description.affiliationUnespDepartment of Biology São Paulo State University UNESP, CEP: 15054-000, São José do Rio Preto /SP
dc.description.affiliationUnespDepartment of Physics São Paulo State University UNESP, CEP: 15054-000, São José do Rio Preto /SP
dc.identifierhttp://dx.doi.org/10.1186/1480-9222-15-10
dc.identifier.citationBiological Procedures Online, v. 15, n. 1, 2013.
dc.identifier.doi10.1186/1480-9222-15-10
dc.identifier.file2-s2.0-84884371369.pdf
dc.identifier.issn1480-9222
dc.identifier.lattes7991082362671212
dc.identifier.lattes4101562077663619
dc.identifier.lattes3313511334783986
dc.identifier.orcid0000-0001-5693-6148
dc.identifier.orcid0000-0002-4731-4977
dc.identifier.scopus2-s2.0-84884371369
dc.identifier.urihttp://hdl.handle.net/11449/76639
dc.identifier.wosWOS:000325075800001
dc.language.isoeng
dc.relation.ispartofBiological Procedures Online
dc.relation.ispartofjcr3.581
dc.relation.ispartofsjr1,352
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCircular dichroism
dc.subjectCloning
dc.subjectHSPC038
dc.subjectMolecular modeling
dc.subjectProtein expression
dc.subjectZNF706 protein
dc.titleExpression, purification and molecular analysis of the human ZNF706 proteinen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.lattes7991082362671212[10]
unesp.author.lattes4101562077663619
unesp.author.lattes3313511334783986[8]
unesp.author.orcid0000-0002-2012-388X[7]
unesp.author.orcid0000-0001-5693-6148[10]
unesp.author.orcid0000-0002-4731-4977[8]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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