Lost and found: Frogs in a biodiversity hotspot rediscovered with environmental DNA

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dc.contributor.authorLopes, Carla Martins [UNESP]
dc.contributor.authorBaêta, Délio [UNESP]
dc.contributor.authorValentini, Alice
dc.contributor.authorLyra, Mariana Lúcio [UNESP]
dc.contributor.authorSabbag, Ariadne Fares [UNESP]
dc.contributor.authorGasparini, João Luiz
dc.contributor.authorDejean, Tony
dc.contributor.authorHaddad, Célio Fernando Basptista [UNESP]
dc.contributor.authorZamudio, Kelly Raquel
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionSPYGEN
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institutionCornell University
dc.date.accessioned2020-12-12T02:21:06Z
dc.date.available2020-12-12T02:21:06Z
dc.date.issued2020-01-01
dc.description.abstractDeclines and extinctions are increasing globally and challenge conservationists to keep pace with biodiversity monitoring. Organisms leave DNA traces in the environment, e.g., in soil, water, and air. These DNA traces are referred to as environmental DNA (eDNA). The analysis of eDNA is a highly sensitive method with the potential to rapidly assess local diversity and the status of threatened species. We searched for DNA traces of 30 target amphibian species of conservation concern, at different levels of threat, using an environmental DNA metabarcoding approach, together with an extensive sequence reference database to analyse water samples from six montane sites in the Atlantic Coastal Forest and adjacent Cerrado grasslands of Brazil. We successfully detected DNA traces of four declined species (Hylodes ornatus, Hylodes regius, Crossodactylus timbuhy, and Vitreorana eurygnatha); two locally disappeared (Phasmahyla exilis and Phasmahyla guttata); and one species that has not been seen since 1968 (putatively assigned to Megaelosia bocainensis). We confirm the presence of species undetected by traditional methods, underscoring the efficacy of eDNA metabarcoding for biodiversity monitoring at low population densities, especially in megadiverse tropical sites. Our results support the potential application of eDNA in conservation biology, to evaluate persistence and distribution of threatened species in surveyed habitats or sites, and improve accuracy of red lists, especially for species undetected over long periods.en
dc.description.affiliationDepartamento de Biodiversidade e Centro de Aquicultura I.B. Universidade Estadual Paulista (UNESP)
dc.description.affiliationSPYGEN
dc.description.affiliationNúcleo em Ecologia e Desenvolvimento Socioambiental de Macaé Universidade Federal do Rio de Janeiro (UFRJ)
dc.description.affiliationDepartment of Ecology and Evolutionary Biology Cornell University
dc.description.affiliationUnespDepartamento de Biodiversidade e Centro de Aquicultura I.B. Universidade Estadual Paulista (UNESP)
dc.identifierhttp://dx.doi.org/10.1111/mec.15594
dc.identifier.citationMolecular Ecology.
dc.identifier.doi10.1111/mec.15594
dc.identifier.issn1365-294X
dc.identifier.issn0962-1083
dc.identifier.scopus2-s2.0-85090160106
dc.identifier.urihttp://hdl.handle.net/11449/200983
dc.language.isoeng
dc.relation.ispartofMolecular Ecology
dc.sourceScopus
dc.subjectamphibians
dc.subjectarchival DNA
dc.subjectbiomonitoring
dc.subjectendangered species
dc.subjectmetabarcoding
dc.subjectpopulation declining
dc.titleLost and found: Frogs in a biodiversity hotspot rediscovered with environmental DNAen
dc.typeTrabalho apresentado em evento
unesp.author.lattes0458077399058762[8]
unesp.author.orcid0000-0002-3277-1913[1]
unesp.author.orcid0000-0002-1427-1878[2]
unesp.author.orcid0000-0001-5829-5479[3]
unesp.author.orcid0000-0002-7863-4965[4]
unesp.author.orcid0000-0001-7277-0950[5]
unesp.author.orcid0000-0001-5107-6206[9]
unesp.author.orcid0000-0002-7044-5764[8]

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Jaboticabal - CAUNESP - Centro de Aquicultura da Unesp