Comparison between histochemical and immunohistochemical methods for diagnosis of sporotrichosis

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dc.contributor.authorMarques, Mariângela Esther Alencar [UNESP]
dc.contributor.authorCoelho, Kunie Iabuki Rabello [UNESP]
dc.contributor.authorSotto, M. N. [UNESP]
dc.contributor.authorBacchi, C. E. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:17:27Z
dc.date.available2014-05-27T11:17:27Z
dc.date.issued1992-01-01
dc.description.abstractAims: To compare the efficacy of histochemical and immunohistochemical methods in detecting forms of Sporothrix schenckii in tissue. Methods: Thirty five cutaneous biopsy specimens from 27 patients with sporotrichosis were stained by histochemical haemotoxylin and eosin, periodic acid Schiff, and Gomori's methenamine silver methods and an immunohistochemical (avidin-biotin complex immunoperoxidase) (ABC) technique associated with a newly produced rabbit polyclonal antibody anti-Sporothrix schenckii. Results: A total of 29 (83%) cases were positive by the ABC method used in association with anti-Sporothrix schenckii rabbit polyclonal antibodies. Histochemical methods, using silver staining, periodic acid Schiff, and conventional haematoxylin and eosin detected 37%, 23%, and 23% of forms of S schenckii, respectively. The ABC technique was significantly more reliable than periodic acid Schiff and silver staining techniques. Conclusions: It is concluded that immunostaining is an easy and rapid method which can efficiently increase the accuracy of the diagnosis of sporotrichosis in human tissue.en
dc.description.affiliationDepartamento de Patologia Faculdade Medicina-UNESP, Botucatu, SP 18610
dc.description.affiliationUnespDepartamento de Patologia Faculdade Medicina-UNESP, Botucatu, SP 18610
dc.format.extent1089-1093
dc.identifierhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC495003/
dc.identifierhttp://dx.doi.org/10.1136/jcp.45.12.1089
dc.identifier.citationJournal of Clinical Pathology, v. 45, n. 12, p. 1089-1093, 1992.
dc.identifier.doi10.1136/jcp.45.12.1089
dc.identifier.file2-s2.0-0026690096.pdf
dc.identifier.issn0021-9746
dc.identifier.lattes7528116925519142
dc.identifier.scopus2-s2.0-0026690096
dc.identifier.urihttp://hdl.handle.net/11449/64210
dc.identifier.wosWOS:A1992KD36800011
dc.language.isoeng
dc.relation.ispartofJournal of Clinical Pathology
dc.relation.ispartofjcr2.894
dc.relation.ispartofsjr1,050
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjecthistochemistry
dc.subjecthuman tissue
dc.subjectimmunohistochemistry
dc.subjectskin biopsy
dc.subjectsporotrichosis
dc.subjectAntibodies, Fungal
dc.subjectHistocytochemistry
dc.subjectImmunohistochemistry
dc.subjectSensitivity and Specificity
dc.subjectSporothrix
dc.subjectSporotrichosis
dc.titleComparison between histochemical and immunohistochemical methods for diagnosis of sporotrichosisen
dc.typeArtigo
unesp.author.lattes7528116925519142
unesp.author.orcid0000-0001-6380-7192[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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