Comparative Study Between Porcine Small Intestinal Submucosa and Buccal Mucosa in a Partial Urethra Substitution in Rabbits

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dc.contributor.authorKawano, Paulo Roberto [UNESP]
dc.contributor.authorFugita, Oscar Eduardo Hidetoshi [UNESP]
dc.contributor.authorYamamoto, Hamilto Akihissa [UNESP]
dc.contributor.authorQuitzan, Juliany Gomes [UNESP]
dc.contributor.authorPadovani, Carlos [UNESP]
dc.contributor.authorAmaro, João Luiz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:38:47Z
dc.date.available2014-05-20T13:38:47Z
dc.date.issued2012-05-01
dc.description.abstractPurpose: Several urethral conditions may require tissue substitution. One collagen-base biomaterial that recently emerged as an option is small intestinal submucosa (SIS). The aim of this study was to compare the results of SIS and buccal mucosa for urethral substitution in rabbits.Materials and Methods: Thirty-six North Folk male rabbits were randomized into three groups. In all animals, a 10 x 5 mm urethral segment was excised, and the urethral defect was repaired using a one-layer SIS patch (group I [GI]); four-layer SIS (group II [GII]); or buccal mucosa (group III [GIII]). Urethrography was performed preoperatively and after 12 weeks. After sacrifice, graft retraction was objectively measured using Scion Image (R) computer analysis and by calculation of ellipse area. The grade of fibrosis, inflammatory reaction, vascular/epithelial regeneration, and collagen III/I ratio were analyzed by hematoxylin/eosin and Picrosirius red staining.Results: Urethrography confirmed a wide urethral caliber without any signs of strictures after surgery. Urethral fistulae was diagnosed in 8.3% of cases (1 animal each group). Average graft shrinkage was 55.2% in GI; 44.2% in GII; and 57.2% in GIII (p < 0.05). The intensity of chronic inflammation, fibrosis, epithelium regeneration, and neovascularization was similar in all groups (p > 0.05). Collagen III/I ratio was higher in GII (GI: 119.6; GII: 257.2 and GIII: 115.0); p < 0.01.Conclusions: The four-layer SIS is more advantageous than the one-layer SIS and buccal mucosa for urethral substitution in rabbits.en
dc.description.affiliationSão Paulo State Univ UNESP, Dept Urol, Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Dept Bioestat, Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Urol, Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Bioestat, Botucatu, SP, Brazil
dc.format.extent427-432
dc.identifierhttp://dx.doi.org/10.1089/end.2011.0174
dc.identifier.citationJournal of Endourology. New Rochelle: Mary Ann Liebert Inc., v. 26, n. 5, p. 427-432, 2012.
dc.identifier.doi10.1089/end.2011.0174
dc.identifier.fileWOS000303403600001.pdf
dc.identifier.issn0892-7790
dc.identifier.lattes9989857854355692
dc.identifier.orcid0000-0001-8411-5822
dc.identifier.urihttp://hdl.handle.net/11449/13447
dc.identifier.wosWOS:000303403600001
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofJournal of Endourology
dc.relation.ispartofjcr2.038
dc.relation.ispartofsjr1,088
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleComparative Study Between Porcine Small Intestinal Submucosa and Buccal Mucosa in a Partial Urethra Substitution in Rabbitsen
dc.typeArtigo
dcterms.licensehttp://www.liebertpub.com/reprints/journal-of-endourologybr--and-part-b-videourology/32/
dcterms.rightsHolderMary Ann Liebert Inc.
unesp.author.lattes9989857854355692[1]
unesp.author.lattes8727897080522289[5]
unesp.author.orcid0000-0002-7719-9682[5]
unesp.author.orcid0000-0003-3745-0761[6]
unesp.author.orcid0000-0001-8411-5822[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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Artigos - Urologia - FMB
Artigos - Bioestatística - IBB