Effects of histone hyperacetylation on the preimplantation development of male and female bovine embryos

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dc.contributor.authorOliveira, Clara S. [UNESP]
dc.contributor.authorSaraiva, Naiara Z. [UNESP]
dc.contributor.authorde Souza, Marcela M. [UNESP]
dc.contributor.authorTetzner, Tatiane A. D. [UNESP]
dc.contributor.authorde Lima, Marina R. [UNESP]
dc.contributor.authorGarcia, Joaquim Mansano [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:30:27Z
dc.date.available2014-05-20T15:30:27Z
dc.date.issued2010-01-01
dc.description.abstractTrichostatin A (TSA) induces histone hyperacetylation by inhibiting histone deacetylases and consequently increasing gene expression. The hypothesis was that TSA supplementation during the in vitro culture (IVC) of bovine embryos would increase the blastocyst rate, particularly in low-quality and female embryos. Oocytes were fertilised separately with X and Y spermatozoa and, 70 h after IVF, the IVC medium was supplemented with 5 nM and 15 nM TSA for 48 or 144 h. Incubation of female embryos with 5 nM and 15 nM TSA resulted in similar increases in acetylated histone H3K9 levels. However, to see comparable effects on acetylated histone H3K9 levels in male embryos, the culture medium needed to be supplemented with 15 nM TSA (as opposed to 5 nM TSA for female embryos). Treatment of male and female embryos with 5 nM TSA for 48 h or female embryos with 5 nM for 144 h had no effect on blastocyst rates, although 15 nM TSA compromised embryonic development. The terminal deoxyribonucleotidyl transferase-mediated dUTP-digoxigenin nick end-labelling (TUNEL) assay revealed increased apoptosis in female embryos treated with 5 nM TSA for 144 h, as well as in male and female embryos treated with 15 nM TSA for 48 h, but this increase in apoptosis was not observed in low-quality embryos. The results of the present study suggest that TSA treatment promotes histone hyperacetylation, but has no beneficial effects on the in vitro production of male and female bovine embryos during preimplantation development.en
dc.description.affiliationSão Paulo State Univ, Fac Ciencias Agr & Vet, Dept Prevent Vet Med & Anim Reprod, BR-14884900 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Fac Ciencias Agr & Vet, Dept Prevent Vet Med & Anim Reprod, BR-14884900 São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1041-1048
dc.identifierhttp://dx.doi.org/10.1071/RD09238
dc.identifier.citationReproduction Fertility and Development. Collingwood: Csiro Publishing, v. 22, n. 6, p. 1041-1048, 2010.
dc.identifier.doi10.1071/RD09238
dc.identifier.issn1031-3613
dc.identifier.lattes2251116139872527
dc.identifier.urihttp://hdl.handle.net/11449/39827
dc.identifier.wosWOS:000279384100016
dc.language.isoeng
dc.publisherCSIRO Publishing
dc.relation.ispartofReproduction, Fertility and Development
dc.relation.ispartofjcr2.105
dc.relation.ispartofsjr0,681
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEpigeneticsen
dc.subjecthistone deacetylase inhibitionen
dc.subjecthistone H3 lys9 acetylationen
dc.subjectimmunocytochemistryen
dc.subjecttrichostatin Aen
dc.subjectTUNEL assayen
dc.titleEffects of histone hyperacetylation on the preimplantation development of male and female bovine embryosen
dc.typeArtigo
dcterms.licensehttp://www.publish.csiro.au/nid/36.htm
dcterms.rightsHolderCsiro Publishing
unesp.author.lattes2251116139872527
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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Artigos - Medicina Veterinária Preventiva e Reprodução Animal - FCAV