Polybitoxins: A group of phospholipases A2 from the venom of the neotropical social wasp paulistinha (Polybia paulista)

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dc.contributor.authorOliveira, Marcia Regina de
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:18:20Z
dc.date.available2014-05-27T11:18:20Z
dc.date.issued1998-01-01
dc.description.abstractThe neotropical wasp Polybia paulista is very aggressive and endemic in south-east Brazil, where it frequently causes stinging accidents. By using gel filtration on Sephadex G-200, followed by ion-exchange chromatography on DEAE-Cellulose under a pH gradient, a group of four toxins (designated as polybitoxins-I, II, lII and IV) presenting phospholipase A2 (PLA2) activities was purified. These toxins are dimeric with mol. wts ranging from 115,000 to 132,000 and formed by different subunits. The four toxins contain very high sugar contents attached to their molecules (22-43% w/w) and presented different values of pH optimum from 7.8 to 9.0; when dissociated, only residual catalytic activities were maintained. The catalytic activities of polybitoxins (from 18 to 771 μmoles/mg per minute) are lower than that of PLA2 from Apis mellifera venom and hornetin from Vespa basalis. The polybitoxins presented a non-linear steady-state kinetic behavior for the hydrolysis of phosphatidylcholine at pH 7.9, compatible with the negative co- operativity phenomena. All of the polybitoxins were very potent direct hemolysins, especially the polybitoxins-III and IV, which are as potent as the lethal toxin from V. basalis and hornetin from Vespa flavitarsus, respectively; polybitoxin-IV presented hemolytic action 20 times higher than that of PLA2 from A. mellifera, 17 times higher than that of neutral PLA2 from Naja nigricolis and about 37 times higher than that of cardiotoxin from Naja naja atra venom.en
dc.description.affiliationInst. of Biosciences of Rio Claro Ctr. the Stud. of Venomous Animals University of São Paulo State, Rio Claro, SP, CEP 13506-900
dc.format.extent189-199
dc.identifierhttp://dx.doi.org/10.1016/S0041-0101(97)00053-6
dc.identifier.citationToxicon, v. 36, n. 1, p. 189-199, 1998.
dc.identifier.doi10.1016/S0041-0101(97)00053-6
dc.identifier.issn0041-0101
dc.identifier.lattes2901888624506535
dc.identifier.scopus2-s2.0-0031925173
dc.identifier.urihttp://hdl.handle.net/11449/65361
dc.language.isoeng
dc.relation.ispartofToxicon
dc.relation.ispartofjcr2.352
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjecthornetin
dc.subjectphospholipase a2
dc.subjectpolybitoxin
dc.subjectsephadex
dc.subjectunclassified drug
dc.subjectanimal cell
dc.subjectanimal tissue
dc.subjectenzyme analysis
dc.subjectenzyme kinetics
dc.subjectenzyme purification
dc.subjecthemolysis
dc.subjecthydrolysis
dc.subjection exchange chromatography
dc.subjectmolecular weight
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectsteady state
dc.subjectwasp
dc.subjectAnimals
dc.subjectErythrocytes
dc.subjectHemolysis
dc.subjectKinetics
dc.subjectMice
dc.subjectMolecular Weight
dc.subjectPhospholipases A
dc.subjectTropical Climate
dc.subjectWasp Venoms
dc.subjectAnimalia
dc.subjectApis mellifera
dc.subjectAtra
dc.subjectInvertebrata
dc.subjectNaja
dc.subjectNaja atra
dc.subjectNaja naja
dc.subjectPolybia
dc.subjectPolybia paulista
dc.subjectVespa basalis
dc.subjectVespidae
dc.titlePolybitoxins: A group of phospholipases A2 from the venom of the neotropical social wasp paulistinha (Polybia paulista)en
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.lattes2901888624506535
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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