In vitro tracking of phospholipase A(2) from snake venom conjugated with magic-sized quantum dots

Página do item simplificado
dc.contributor.authorDias, Edigar H. V.
dc.contributor.authorPereira, Deborah F. C.
dc.contributor.authorSousa, Bruna B. de [UNESP]
dc.contributor.authorMatias, Mariana S.
dc.contributor.authorQueiroz, Mayara R. de
dc.contributor.authorSantiago, Fernanda M.
dc.contributor.authorSilva, Anielle C. A.
dc.contributor.authorDantas, Noelio O.
dc.contributor.authorSantos-Filho, Norival Alves [UNESP]
dc.contributor.authorOliveira, Fabio de
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Nacl Ciencia & Tecnol Nanobiofarmaceut N Bio
dc.date.accessioned2019-10-04T11:56:26Z
dc.date.available2019-10-04T11:56:26Z
dc.date.issued2019-02-01
dc.description.abstractPhospholipases A(2) represent a family of enzymes with important application in medicine. However, direct tracking is difficult due to the absence of a stable, effective and specific marker for these enzymes. Magic-sized quantum dots (MSQDs) are inorganic semiconducting nanocrystals with unique physical properties. They have the ability to conjugate to proteins, making them excellent markers for biological systems. In this work, we labelled phospholipase A(2) from Bothrops alternatus snake venom with Cadmium selenide (CdSe)/cadmium sulphate (CdS) MSQDs-a biocompatible and luminescent probe. Bioconjugation was confirmed using infrared spectra and fluorescence microscopy, which demonstrated that the CdSe/CdS MSQDs interact with phospholipase A(2) without interfering with its activity. This probe may be an important tool for the elucidation of many biological mechanisms, because it allows the pathway of phospholipase A(2) to be tracked from its entry through the plasma membrane until its incorporation into the nucleus of myoblasts. (C) 2018 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Fed Uberlandia, Inst Genet & Bioquim, Uberlandia, MG, Brazil
dc.description.affiliationUniv Fed Uberlandia, Inst Ciencias Biomed, Uberlandia, MG, Brazil
dc.description.affiliationUniv Fed Uberlandia, Inst Fis, Uberlandia, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Quim, Araraquara, SP, Brazil
dc.description.affiliationInst Nacl Ciencia & Tecnol Nanobiofarmaceut N Bio, Belo Horizonte, MG, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipMinisterio de Ciencias e Tecnologia (MCT) of Brazil
dc.format.extent461-468
dc.identifierhttp://dx.doi.org/10.1016/j.ijbiomac.2018.10.185
dc.identifier.citationInternational Journal Of Biological Macromolecules. Amsterdam: Elsevier Science Bv, v. 122, p. 461-468, 2019.
dc.identifier.doi10.1016/j.ijbiomac.2018.10.185
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/11449/184287
dc.identifier.wosWOS:000456226700051
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Journal Of Biological Macromolecules
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectLuminescent probe
dc.subjectPhospholipase A(2)
dc.subjectMagic-sized quantum dot
dc.titleIn vitro tracking of phospholipase A(2) from snake venom conjugated with magic-sized quantum dotsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.

Arquivos

Coleções

Artigos