TNF-alpha, H2O2 and NO response of peritoneal macrophages to Yersinia enterocolitica O : 3 derivatives

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dc.contributor.authorCarlos, Iracilda Zeppone [UNESP]
dc.contributor.authorMonnazzi, LGS
dc.contributor.authorFalcao, D. P.
dc.contributor.authorde Medeiros, BMM
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:22Z
dc.date.available2014-05-20T13:24:22Z
dc.date.issued2004-02-01
dc.description.abstractIn this study, the effect of Yersinia derivatives on nitric oxide (NO), hydrogen peroxide (H2O2) and tumor necrosis factor-alpha (TNF-alpha) production by murine peritoneal macrophages was investigated. Addition of lipopolysaccharide (LPS) to the macrophage culture resulted in NO production that was dose dependent. on the other hand, bacterial cellular extract (CE) and Yersinia outer proteins (Yops) had no effect on NO production. The possible inhibitory effect of Yops on macrophage cultures stimulated with LPS was investigated. Yops partially inhibited NO production (67.4%) when compared with aminoguanidine. The effects of Yersinia derivatives on H2O2 production by macrophages were similar to those on NO production. LPS was the only derivative that stimulated H2O2 release in a dose-dependent manner. All Yersinia derivatives provoked the production of TNF-alpha, but LPS had the strongest effect, as observed for NO production. CE and Yops stimulated TNF-alpha production to a lesser extent than LPS. The results indicate the possibility that in vivo Yops may aid the evasion of the bacteria from the host defense mechanism by impairing the secretion of NO by macrophages. (C) 2003 Elsevier SAS. All rights reserved.en
dc.description.affiliationUNESP, Fac Ciências Farmaceut Araraquara, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUNESP, Fac Ciências Farmaceut Araraquara, Dept Ciências Biol, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Farmaceut Araraquara, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Farmaceut Araraquara, Dept Ciências Biol, BR-14801902 Araraquara, SP, Brazil
dc.format.extent207-212
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2003.10.012
dc.identifier.citationMicrobes and Infection. Amsterdam: Elsevier B.V., v. 6, n. 2, p. 207-212, 2004.
dc.identifier.doi10.1016/j.micinf.2003.10.012
dc.identifier.issn1286-4579
dc.identifier.lattes1730146818754269
dc.identifier.urihttp://hdl.handle.net/11449/7528
dc.identifier.wosWOS:000220275200010
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofMicrobes and Infection
dc.relation.ispartofjcr2.924
dc.relation.ispartofsjr1,205
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectYersinia enterocoliticapt
dc.subjectmacrophagespt
dc.subjectnitric oxidept
dc.subjecthydrogen peroxidept
dc.subjectTNF-alphapt
dc.titleTNF-alpha, H2O2 and NO response of peritoneal macrophages to Yersinia enterocolitica O : 3 derivativesen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes1730146818754269
unesp.author.orcid0000-0002-0084-3468[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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