Mutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assays

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dc.contributor.authorResende, Flavia Aparecida [UNESP]
dc.contributor.authorBarbosa, Lilian Cristina
dc.contributor.authorTavares, Denise Crispim
dc.contributor.authorde Camargo, Mariana Santoro [UNESP]
dc.contributor.authorde Souza Rezende, Karen Cristina
dc.contributor.authorde Andrade e Silva, Marcio Luis
dc.contributor.authorVaranda, Eliana Aparecida [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Franca
dc.date.accessioned2014-05-20T13:24:19Z
dc.date.available2014-05-20T13:24:19Z
dc.date.issued2012-10-31
dc.description.abstractBackground: The dibenzylbutyrolactone lignan (-)-hinokinin (HK) was derived by partial synthesis from (-)- cubebin, isolated from the dry seeds of the pepper, Piper cubeba. Considering the good trypanosomicidal activity of HK and recalling that natural products are promising starting points for the discovery of novel potentially therapeutic agents, the aim of the present study was to investigate the (anti) mutagenic/genotoxic activities of HK.Methods: The mutagenic/genotoxic activities were evaluated by the Ames test on Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and the comet assay, so as to assess the safe use of HK in the treatment of Chagas' disease. The antimutagenic /antigenotoxic potential of HK were also tested against the mutagenicity of a variety of direct and indirect acting mutagens, such as 4- nitro-o-phenylenediamine (NOPD), sodium azide (SA), mitomycin C (MMC), benzo[a] pyrene (B[a] P), aflatoxin B-1 (AFB(1)), 2-aminoanthracene (2-AA) and 2-aminofluorene (2-AF), by the Ames test, and doxorubicin (DXR) by the comet assay.Results: The mutagenicity/genotoxicity tests showed that HK did not induce any increase in the number of revertants or extent of DNA damage, demonstrating the absence of mutagenic and genotoxic activities. on the other hand, the results on the antimutagenic potential of HK showed a strong inhibitory effect against some direct and indirect-acting mutagens.Conclusions: Regarding the use of HK as an antichagasic drug, the absence of mutagenic effects in animal cell and bacterial systems is encouraging. In addition, HK may be a new potential antigenotoxic /antimutagenic agent from natural sources. However, the protective activity of HK is not general and varies with the type of DNA damage-inducing agent used.en
dc.description.affiliationUNESP Univ Estadual Paulista Julio Mesquita Filho, Fac Ciencias Farmaceut Araraquara, Dept Ciencias Biol, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUniv Franca, BR-14404600 Franca, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista Julio Mesquita Filho, Fac Ciencias Farmaceut Araraquara, Dept Ciencias Biol, BR-14801902 Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent10
dc.identifierhttp://dx.doi.org/10.1186/1472-6882-12-203
dc.identifier.citationBmc Complementary and Alternative Medicine. London: Biomed Central Ltd., v. 12, p. 10, 2012.
dc.identifier.doi10.1186/1472-6882-12-203
dc.identifier.fileWOS000313500600001.pdf
dc.identifier.issn1472-6882
dc.identifier.lattes7501930236496670
dc.identifier.urihttp://hdl.handle.net/11449/7506
dc.identifier.wosWOS:000313500600001
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofBMC Complementary and Alternative Medicine
dc.relation.ispartofjcr2.109
dc.relation.ispartofsjr0,858
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectHinokininen
dc.subjectAmes testen
dc.subjectComet assayen
dc.subjectMutagenicityen
dc.subjectAntimutagenicityen
dc.titleMutagenicity and antimutagenicity of (-)-hinokinin a trypanosomicidal compound measured by Salmonella microsome and comet assaysen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
unesp.author.lattes7501930236496670
unesp.author.orcid0000-0002-9432-5919[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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