Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation

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dc.contributor.authorde Lima Souza, Hilton Marcelo
dc.contributor.authorSette, Lara Durães [UNESP]
dc.contributor.authorda Mota, Adolfo José
dc.contributor.authordo Nascimento Neto, Joaquim Ferreira
dc.contributor.authorRodrigues, André [UNESP]
dc.contributor.authorde Oliveira, Tássio Brito [UNESP]
dc.contributor.authorde Oliveira, Fernando Mendes
dc.contributor.authorde Oliveira, Luiz Antônio
dc.contributor.authordos Santos Barroso, Hileia
dc.contributor.authorZanotto, Sandra Patricia
dc.contributor.institutionEscola Superior de Ciências da Saúde
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionDivisão deBiotecnologia
dc.contributor.institutionInstituto Nacional de Pesquisas da Amazônia (INPA)
dc.date.accessioned2018-12-11T17:08:12Z
dc.date.available2018-12-11T17:08:12Z
dc.date.issued2016-12-01
dc.description.abstractFilamentous fungi were isolated from contaminated sediment samples in the Amazon region of Brazil to select species with potential for benzo(a)pyrene (BaP) degradation, a polycyclic aromatic hydrocarbon (PAH) with high molecular weight and known for its mutagenic and carcinogenic properties. The isolates were submitted to biodegradability test using 2.6-dichlorophenol indophenol (DCPIP), gallic acid reaction, and evaluation of BaP influence in the enzymatic (ligninolytic) activity. The selected fungi were submitted to the taxonomic identification and used in biodegradation assays, which were carried out using gas chromatography coupled to mass spectrometry (GC-MS). A qualitative analysis of the presence of BaP metabolites that have recently been reported in literature was also performed by GC-MS. A total of 146 fungal isolates were recovered. Among them, 63.7 % were positive for the redox indicator DCPIP. From these isolates, 22.6 % showed positive responses to the gallic acid reaction. In enzymatic tests, the fungi Megasporoporia sp. S47 and unidentified Sordariales S69 presented highest activities of laccase and manganese peroxidase in the presence of BaP. Additionally, the white-rot fungus Megasporoporia sp. S47 showed better performance in BaP degradation (54 %). Therefore, Megasporoporia sp. S47, obtained from an environment with considerable PAH contamination, was selected as a promising genetic resource for application in new studies related to enzyme production and characterization and BaP degradation optimization.en
dc.description.affiliationUniversidade do Estado do Amazonas Escola Superior de Ciências da Saúde, Av. Carvalho Leal, 1777
dc.description.affiliationUniversidade Estadual Paulista “Júlio de Mesquita Filho” Campus de Rio Claro Departamento deBioquímica e Microbiologia Aplicada IB/UNESP, Av. 24-a, 1515
dc.description.affiliationUniversidade Federal do Amazonas Departamento de Ciências Fundamentais e DesenvolvimentoAgrícola (DCFDA) / Faculdade de Ciências Agrárias (FCA) e Centro de Apoio Multidisciplinar Divisão deBiotecnologia, Av. General Rodrigo Octávio Jordão Ramos, 3000
dc.description.affiliationInstituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo, 2.936
dc.description.affiliationUnespUniversidade Estadual Paulista “Júlio de Mesquita Filho” Campus de Rio Claro Departamento deBioquímica e Microbiologia Aplicada IB/UNESP, Av. 24-a, 1515
dc.identifierhttp://dx.doi.org/10.1007/s11270-016-3101-y
dc.identifier.citationWater, Air, and Soil Pollution, v. 227, n. 12, 2016.
dc.identifier.doi10.1007/s11270-016-3101-y
dc.identifier.file2-s2.0-85003554296.pdf
dc.identifier.issn1573-2932
dc.identifier.issn0049-6979
dc.identifier.lattes5969653098289575
dc.identifier.scopus2-s2.0-85003554296
dc.identifier.urihttp://hdl.handle.net/11449/173889
dc.language.isoeng
dc.relation.ispartofWater, Air, and Soil Pollution
dc.relation.ispartofsjr0,589
dc.relation.ispartofsjr0,589
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBenzo(a)pyrene
dc.subjectBiodegradation
dc.subjectFungi
dc.subjectLigninolytic enzymes
dc.subjectPolycyclic aromatic hydrocarbons
dc.titleFilamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradationen
dc.typeArtigo
unesp.author.lattes5969653098289575
unesp.author.lattes8538509657578022[5]
unesp.author.orcid0000-0002-4164-9362[5]

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