Development and transferability of microsatellite markers in Habenaria nuda and H. repens (Orchidaceae)

Página do item simplificado
dc.contributor.authorVale, Aline Amalia do
dc.contributor.authorLau, Bruna Ladeira
dc.contributor.authorSantos Leal, Barbara Simoes [UNESP]
dc.contributor.authorNogueira Batista, Joao Aguiar
dc.contributor.authorBorba, Eduardo Leite
dc.contributor.authorProite, Karina
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do ABC (UFABC)
dc.date.accessioned2018-11-26T16:32:38Z
dc.date.available2018-11-26T16:32:38Z
dc.date.issued2016-03-01
dc.description.abstractWe report here the first set of microsatellite markers developed for species of Habenaria. A set of microsatellite markers for Habenaria nuda Lindl. and H. repens Nutt. were isolated, characterized, and tested for cross-amplification between these congeneric species. These species constitute two of the many existing complexes of the genus Habenaria. A total of nine markers were isolated from both species and characterized in two populations of each, with some of the markers being successfully cross-amplified. The numbers of alleles per locus ranged from four to 12 in H. nuda, and the mean numbers of alleles per locus were 6.0 and 6.14 in each of the populations analyzed. The observed (Ho) and expected (He) heterozygosities within the two populations varied from 0.294 to 0.850 and from 0.517 to 0.851, respectively. The numbers of alleles per locus ranged from four to seven in H. repens, and the mean numbers of alleles per locus were 2.8 and 4.0 in each of the populations analyzed. The Ho ranged from 0.000 to 0.563, and the He ranged from 0.000 to 0.666. The polymorphism levels found indicate these markers as powerful tools for future population genetic analyses in these and related species and could be used to address ecological, evolutionary, and taxonomic questions.en
dc.description.affiliationUniv Fed Minas Gerais, Dept Bot, Caixa Postal 486, BR-31270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Ecol, Campus Rio Claro, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUniv Fed ABC, Ctr Ciencias Nat & Humanas, Campus Sao Bernardo Campo, BR-09606070 Sao Bernardo Do Campo, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Ecol, Campus Rio Claro, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCAPES: 02805/09-0
dc.description.sponsorshipIdCNPq: PQ2
dc.description.sponsorshipIdCNPq: PQ1B
dc.format.extent387-392
dc.identifierhttp://dx.doi.org/10.1007/s40415-015-0214-2
dc.identifier.citationBrazilian Journal Of Botany. Sao Paulo: Soc Botanica Sao Paulo, v. 39, n. 1, p. 387-392, 2016.
dc.identifier.doi10.1007/s40415-015-0214-2
dc.identifier.fileWOS000373897100040.pdf
dc.identifier.issn1806-9959
dc.identifier.urihttp://hdl.handle.net/11449/161410
dc.identifier.wosWOS:000373897100040
dc.language.isoeng
dc.publisherSoc Botanica Sao Paulo
dc.relation.ispartofBrazilian Journal Of Botany
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectGenetic diversity
dc.subjectHabenaria
dc.subjectMicrosatellite markers
dc.subjectSSR
dc.subjectTransferability
dc.titleDevelopment and transferability of microsatellite markers in Habenaria nuda and H. repens (Orchidaceae)en
dc.typeArtigo
dcterms.rightsHolderSoc Botanica Sao Paulo
unesp.author.orcid0000-0001-6331-3766[1]
unesp.author.orcid0000-0003-4640-0942[4]

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000373897100040.pdf
Tamanho:
359.79 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Artigos - Ecologia - IBRC