Nitric oxide of the supraoptic nucleus influences the salivary secretion, sodium renal excretion, urinary volume and arterial blood pressure induced by pilocarpine

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dc.contributor.authorSaad, W. A.
dc.contributor.authorGutierrez, L. I.
dc.contributor.authorGuarda, I. F. M. S.
dc.contributor.authorCamargo, L. A. D.
dc.contributor.authorSantos, T. A. F. B. dos
dc.contributor.authorSimoes, S.
dc.contributor.authorGuarda, R. S.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de Taubaté (UNITAU)
dc.contributor.institutionCentro Universitário de Araraquara (UNIARA)
dc.contributor.institutionHospital 9 de Juho
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:45:47Z
dc.date.available2014-05-20T13:45:47Z
dc.date.issued2004-02-13
dc.description.abstractMale Holtzman rats weighting 200-250 g were anesthetized with zoletil 50 mg/Kg (tiletamine chloridrate 125.0 mg and zolazepan chloridrate 125.0 mg) into quadriceps muscle and stainless steel cannulas were implanted into their supraoptic nucleus (SON). We investigated the effects of the injection into the supraoptic nucleus (SON) of FK 409, a nitric oxide donor, and N(W-)nitro-L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor (NOS), on the salivary secretion, arterial blood pressure, sodium excretion and urinary volume induced by pilocarpine, which was injected into SON. The drugs were injected in 0.5 mul volume over 30-60 s. Controls was injected with a similar volume of 0.15 M NaCl. FK 409 and L-NAME were injected at doses of 20 mug/0.5 mul and 40 mug/0.5 mul. respectively. The amount of saliva secretion was studied over a five-minute period after injection of pilocarpine into SON. Injection of pilocarpine (10, 20, 40, 80, 160 mug/mul) into SON produced a dose-dependent increase in salivary secretion. L-NAME was injected into SON prior to the injection of pilocarpine into SON, producing an increase in salivary secretion due to the effect of pilocarpine. FK 409 injected into SON attenuating the increase in salivary secretion induced by pilocarpine. Mean arterial pressure (MAP) increase after injections of pilocarpine into the SON. L-NAME injected into the SON prior to injection of pilocarpine into SON increased the MAP. FK 409 injected into the SON prior to pilocarpine attenuated the effect of pilocarpine on MAP. Pilocarpine (0.5 mumol/0.5 mul) injected into the SON induced an increase in sodium and urinary excretion. L-NAME injected prior to pilocarpine into the SON increased the urinary sodium excretion and urinary volume induced by pilocarpine. FK 409 injected prior to pilocarpine into the SON decreased the sodium excretion and urinary volume induced by pilocarpine. All these roles of pilocarpine depend on the release of nitric oxide into the SON. In summary the present results show: a) SON is involved in pilocarpine-induced salivation; b) that mechanism involves increase in MAP, sodium excretion and urinary volume. (C) 2003 Elsevier B.V. All rights reserved.en
dc.description.affiliationPaulista State Univ, UNESP, Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil
dc.description.affiliationUNITAU, Dept Dent, Taubate, SP, Brazil
dc.description.affiliationUNIARA, Dept Exact & Nat Sci, Araraquara, SP, Brazil
dc.description.affiliation9 Julho Med Hosp, Dept Anesthesiol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Surg, São Paulo, Brazil
dc.description.affiliationUnespPaulista State Univ, UNESP, Sch Dent, Dept Physiol & Pathol, BR-14801903 Araraquara, SP, Brazil
dc.format.extent1593-1603
dc.identifierhttp://dx.doi.org/10.1016/j.lfs.2003.08.027
dc.identifier.citationLife Sciences. Oxford: Pergamon-Elsevier B.V., v. 74, n. 13, p. 1593-1603, 2004.
dc.identifier.doi10.1016/j.lfs.2003.08.027
dc.identifier.issn0024-3205
dc.identifier.urihttp://hdl.handle.net/11449/16138
dc.identifier.wosWOS:000188539700003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofLife Sciences
dc.relation.ispartofjcr3.234
dc.relation.ispartofsjr1,071
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectpilocarpinept
dc.subjectL-NAMEpt
dc.subjectFK 409 supraoptic nucleuspt
dc.subjectsalivationpt
dc.subjectarterial pressurept
dc.subjectsodium excretionpt
dc.titleNitric oxide of the supraoptic nucleus influences the salivary secretion, sodium renal excretion, urinary volume and arterial blood pressure induced by pilocarpineen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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