Maternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insult

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dc.contributor.authorda Silva, Flávia R. M.
dc.contributor.authorZapaterini, Joyce R. [UNESP]
dc.contributor.authorGrassi, Tony F. [UNESP]
dc.contributor.authorBidinotto, Lucas T. [UNESP]
dc.contributor.authorFernandes, Ana Angélica H. [UNESP]
dc.contributor.authorBarbisan, Luis F. [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionBarretos Cancer Hospital
dc.contributor.institutionBarretos School of Health Sciences
dc.date.accessioned2023-07-29T13:50:41Z
dc.date.available2023-07-29T13:50:41Z
dc.date.issued2023-01-01
dc.description.abstractWe evaluated the effects of prenatal and postnatal dietary zinc (Zn) deficiency or supplementation on mammary gland morphology and on acute response to 7,12-dimethylbenzanthracene (DMBA) in pubertal female rats. On gestational day 10 (GD 10), rat dams were allocated randomly into three experimental groups of 10: a Zn-adequate diet group (ZnA) fed 35 mg Zn/kg chow, a Zn-deficient diet group (ZnD) fed 3 mg ZN/kg chow and a Zn-supplemented diet group (ZnS) fed 180 mg Zn/kg chow. After weaning, female offspring were fed the same diet as their dams until postnatal day 53 (PND 53). All animals received a single 50 mg/kg dose of DMBA on PND 51 and were euthanized on PND 53. Female ZnD offspring exhibited significantly less weight gain compared to the ZnA group and reduced mammary gland development compared to the ZnD and ZnA groups. By PND 53, the Ki-67 labeling index in mammary gland epithelial cells was significantly greater for the ZnS group than for the ZnA and ZnD groups. Apoptosis and ER-α indices did not differ among groups. The ZnD group exhibited significantly increased lipid hydroperoxide (LOOH) levels and decreased catalase and glutathione peroxidase (GSH-Px) activity compared to the ZnA and ZnS groups. The ZnS group exhibited significantly reduced superoxide dismutase (SOD) activity compared to the ZnA and ZnS groups. We observed atypical ductal hyperplasia in the mammary gland of female ZnS group offspring compared to the ZnA and ZnD groups and decreased expression of the Api5 and Ercc1 genes related to apoptosis inhibition and DNA damage repair, respectively. Both the Zn-deficient and Zn-supplemented diet exerted adverse effects on offspring mammary gland morphology and acute response to DMBA.en
dc.description.affiliationFaculty of Pharmaceutical Sciences Campinas University, SP
dc.description.affiliationDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University, SP
dc.description.affiliationDepartment of Pathology School of Medicine São Paulo State University
dc.description.affiliationMolecular Oncology Research Center Barretos Cancer Hospital, SP
dc.description.affiliationDr. Paulo Prata-FACISB Barretos School of Health Sciences, SP
dc.description.affiliationDepartment of Chemistry and Biochemistry Institute of Biosciences São Paulo State University, SP
dc.description.affiliationUnespDepartment of Structural and Functional Biology Institute of Biosciences São Paulo State University, SP
dc.description.affiliationUnespDepartment of Pathology School of Medicine São Paulo State University
dc.description.affiliationUnespDepartment of Chemistry and Biochemistry Institute of Biosciences São Paulo State University, SP
dc.identifierhttp://dx.doi.org/10.1080/10520295.2023.2196092
dc.identifier.citationBiotechnic and Histochemistry.
dc.identifier.doi10.1080/10520295.2023.2196092
dc.identifier.issn1473-7760
dc.identifier.issn1052-0295
dc.identifier.scopus2-s2.0-85152373801
dc.identifier.urihttp://hdl.handle.net/11449/248680
dc.language.isoeng
dc.relation.ispartofBiotechnic and Histochemistry
dc.sourceScopus
dc.subject7,12-dimethylbenzanthracene
dc.subjectDevelopment
dc.subjectmammary gland
dc.subjectrats
dc.subjectzinc
dc.titleMaternal dietary zinc status alters offspring female mammary gland development and response to acute 7,12-dimethylbenzanthracene insulten
dc.typeArtigo

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