Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis

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dc.contributor.authorBannwart, Camila Ferreira [UNESP]
dc.contributor.authorMartins, Rosana A Rodrigues [UNESP]
dc.contributor.authorNakaira-Takahashi, Érika [UNESP]
dc.contributor.authorDias-Melício, Luciane A. [UNESP]
dc.contributor.authorSoares, Ângela MV Campos [UNESP]
dc.contributor.authorPeraçoli, Maria Terezinha S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:50:44Z
dc.date.available2014-05-20T13:50:44Z
dc.date.issued2010-11-01
dc.description.abstractInterleukin (IL)-15 is a pleiotropic cytokine that regulates the proliferation and survival of many cell types. IL-15 is produced by monocytes and macrophages against infectious agents and plays a pivotal role in innate and adaptive immune responses. This study analyzed the effect of IL-15 on fungicidal activity, oxidative metabolism and cytokine production by human monocytes challenged in vitro with Paracoccidioides brasiliensis (Pb18), the agent of paracoccidioidomycosis. Peripheral blood monocytes were pre-incubated with IL-15 and then challenged with Pb18. Fungicidal activity was assessed by viable fungi recovery from cultures after plating on brain-heart infusion-agar. Superoxide anion (O2-), hydrogen peroxide (H2O2), tumour necrosis factor-alpha (TNF-α), IL-6, IL-15 and IL-10 production by monocytes were also determined. IL-15 enhanced fungicidal activity against Pb18 in a dose-dependent pattern. This effect was abrogated by addition of anti-IL-15 monoclonal antibody. A significant stimulatory effect of IL-15 on O2- and H2O2 release suggests that fungicidal activity was dependent on the activation of oxidative metabolism. Pre-treatment of monocytes with IL-15 induced significantly higher levels of TNF-α, IL-10 and IL-15 production by cells challenged with the fungus. These results suggest a modulatory effect of IL-15 on pro and anti-inflammatory cytokine production, oxidative metabolism and fungicidal activity of monocytes during Pb18 infection.en
dc.description.affiliationUniversidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e Imunologia
dc.description.affiliationUnespUniversidade Estadual Paulista Instituto de Biociências Departamento de Microbiologia e Imunologia
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 05/54283-7
dc.description.sponsorshipIdFAPESP: 03/13743-0
dc.format.extent866-872
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762010000700005
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 7, p. 866-872, 2010.
dc.identifier.doi10.1590/S0074-02762010000700005
dc.identifier.fileS0074-02762010000700005.pdf
dc.identifier.issn0074-0276
dc.identifier.scieloS0074-02762010000700005
dc.identifier.urihttp://hdl.handle.net/11449/18119
dc.identifier.wosWOS:000284778900005
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectIL-15en
dc.subjectParacoccidioides brasiliensisen
dc.subjectmonocytesen
dc.subjecthydrogen peroxideen
dc.subjectfungicidal activityen
dc.subjectcytokinesen
dc.titleInterleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensisen
dc.typeArtigo
unesp.author.orcid0000-0002-0936-9512[6]
unesp.author.orcid0000-0003-1962-9901[5]
unesp.author.orcid0000-0001-9254-2074[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB