Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability

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dc.contributor.authorCarneiro, Joao A.M. [UNESP]
dc.contributor.authorCanisso, Igor F.
dc.contributor.authorBandeira, R. S. [UNESP]
dc.contributor.authorScheeren, V. F.C. [UNESP]
dc.contributor.authorFreitas-Dell'Aqua, Camila P. [UNESP]
dc.contributor.authorAlvarenga, Marco A. [UNESP]
dc.contributor.authorPapa, Frederico O. [UNESP]
dc.contributor.authorDell'Aqua, Jose A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Illinois Urbana-Champaign
dc.date.accessioned2018-12-11T17:36:03Z
dc.date.available2018-12-11T17:36:03Z
dc.date.issued2018-05-01
dc.description.abstractThis study aimed to evaluate the antioxidant properties of coenzyme Q10 (CoQ10) during cryopreservation of semen obtained from stallions having good and bad semen freezing ability (GFA vs. BFA, respectively). Forty ejaculates (n = 20 stallions) were split into five centrifugation and five freezing extenders containing different concentrations of CoQ10 (0, 25, 50, 75 and 100 μmols/L). If CoQ10 was added to the centrifugation extender, the freezing extender had no CoQ10 added; similarly, if CoQ10 was added to the freezing extender, the centrifugation extender had no CoQ10. Semen cryopreserved on extenders containing no CoQ10 served as the control. After post-thaw total sperm motility (TM) assessments, the stallions were classified as GFA (i.e., decrease of ≤25% in TM, n = 7) or BFA (i.e., decrease of ≥40% in TM, n = 5). Stallions not fitting (n = 8) this enrollment criteria had samples discarded. After that, two straws for each extender were thawed at 37 °C for 30 s; one straw was immediately used for evaluation of sperm kinetics, plasma membrane integrity, non-capacitated spermatozoa, reactive oxygen species production, mitochondrial activity and lipid peroxidation. The second straw was kept at 37 °C for 30 min and subjected to the same assessments. Expectedly, sperm motility parameters were significantly lower for stallions with BFA. There were no effects of CoQ10 concentration or time for all parameters evaluated in the group with GFA when compared with the control extender (p > 0.05), except lipid peroxidation (p < 0.05). However, stallions with BFA had improved sperm parameters for samples processed with extenders containing CoQ10 (particularly 75 μmols/L) (p < 0.05), except for the reactive oxygen species production and mitochondrial potential (T0) in which there were no differences between the groups (p > 0.05). In summary, 75 μmols/L appears to be the optimal dose of Co-Q10, particularly, when added to the centrifugation extender.en
dc.description.affiliationSao Paulo State University (UNESP) School of Veterinary Medicine and Animal Science
dc.description.affiliationDepartment of Veterinary Clinical Medicine College of Veterinary Medicine University of Illinois Urbana-Champaign
dc.description.affiliationUnespSao Paulo State University (UNESP) School of Veterinary Medicine and Animal Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2015/25638-3
dc.format.extent107-118
dc.identifierhttp://dx.doi.org/10.1016/j.anireprosci.2018.02.020
dc.identifier.citationAnimal Reproduction Science, v. 192, p. 107-118.
dc.identifier.doi10.1016/j.anireprosci.2018.02.020
dc.identifier.file2-s2.0-85042618261.pdf
dc.identifier.issn0378-4320
dc.identifier.scopus2-s2.0-85042618261
dc.identifier.urihttp://hdl.handle.net/11449/179619
dc.language.isoeng
dc.relation.ispartofAnimal Reproduction Science
dc.relation.ispartofsjr0,704
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAntioxidant
dc.subjectCryodamage
dc.subjectHorse
dc.subjectOxidative stress
dc.subjectSemen freezing
dc.titleEffects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing abilityen
dc.typeArtigo

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