Antioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae)

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dc.contributor.authorLopes, Andrey P.
dc.contributor.authorBagatela, Bianca S.
dc.contributor.authorRosa, Paulo C. P.
dc.contributor.authorNanayakkara, Dhammika N. P.
dc.contributor.authorCarlos Tavares Carvalho, José
dc.contributor.authorMaistro, Edson Luis [UNESP]
dc.contributor.authorBastos, Jairo K.
dc.contributor.authorPerazzo, Fábio F.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversity of Mississippi
dc.contributor.institutionUniversidade Federal do Amapá (UNIFAP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:28:39Z
dc.date.available2014-05-27T11:28:39Z
dc.date.issued2013-03-11
dc.description.abstractThe crude ethanolic extract from aerial parts of Pothomorphe umbellata L. (Piperaceae) and fractions obtained by partitions sequentially among water-methanol, methylene chloride, and ethyl acetate, as well as the major constituent, 4-nerolidylcatechol, were, respectively, evaluated and evidenced for antioxidant and cytotoxic effects through fluorometric microplate and microculture tetrazolium assays in HL-60 cells. The crude ethanolic extract demonstrated the preeminent antioxidant activity (IC50 = 1.2 μg/mL) against exogenous cytoplasmic reactive oxygen species, followed by the water-methanolic (IC50 = 4.5 μg/mL), methylene chloride (IC 50 = 5.9 g/mL), ethyl acetate (IC50 = 8.0 g/mL), 4-nerolidylcatechol (IC50 = 8.6 g/mL), and the sterol fractions (IC50 > 12.5 μg/mL). Vitamin C, the positive control used in this assay, presented IC50 value equivalent to 1.7 μg/mL. 4-Nerolidylcatechol (IC50 = 0.4 μg/mL) and methylene chloride fraction (IC50 = 2.3 μg/mL) presented considerable cytotoxicity probably because of the presence of an o-quinone, an auto-oxidation by product of the catechol. Polar compounds, present in the ethanol extract, appear to increase the solubility and stability of the major active constituent, acting synergistically with 4-nerolidylcatechol, improving its pharmacokinetic parameters and increasing significantly its antioxidant activity which, in turn, suggests that the aqueous-ethanolic extract, used in folklore medicine, is safe and effective. © 2013 Andrey P. Lopes et al.en
dc.description.affiliationDepartamento de Ciências Exatas e da Terra Instituto de Ciências Ambientais Universidade Federal de São Paulo, São Nicolau Street 210, 09972-270 Diadema, SP
dc.description.affiliationNational Center for Natural Products Research Research Institute of Pharmaceutical Sciences University of Mississippi, Oxford, MS 38677
dc.description.affiliationLaboratório de Pesquisa em Fármacos Universidade Federal Do Amapá, 68902-280 Macapá, AP
dc.description.affiliationDepartamento de Fonoaudiologia Faculdade de Filosofia e Ciências Universidade Estadual Paulista, 17525-900 Marília, SP
dc.description.affiliationDepartamento de Ciências Farmacêuticas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, 14040-903 Ribeirão Preto, SP
dc.description.affiliationUnespDepartamento de Fonoaudiologia Faculdade de Filosofia e Ciências Universidade Estadual Paulista, 17525-900 Marília, SP
dc.identifierhttp://dx.doi.org/10.1155/2013/206581
dc.identifier.citationBioMed Research International, v. 2013.
dc.identifier.doi10.1155/2013/206581
dc.identifier.file2-s2.0-84874586881.pdf
dc.identifier.issn2314-6133
dc.identifier.issn2314-6141
dc.identifier.lattes4787521613038315
dc.identifier.orcid0000-0003-0757-7876
dc.identifier.scopus2-s2.0-84874586881
dc.identifier.urihttp://hdl.handle.net/11449/74820
dc.identifier.wosWOS:000313686800001
dc.language.isoeng
dc.relation.ispartofBioMed Research International
dc.relation.ispartofjcr2.583
dc.relation.ispartofsjr0,935
dc.relation.ispartofsjr0,935
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subject4 nerolidylcatechol
dc.subjectacetic acid ethyl ester
dc.subjectalcohol
dc.subjectcatechol derivative
dc.subjectdichloromethane
dc.subjectplant extract
dc.subjectPothomorphe umbellata plant extract
dc.subjectsterol
dc.subjectunclassified drug
dc.subjectantioxidant activity
dc.subjectcontrolled study
dc.subjectcytotoxicity
dc.subjectdrug solubility
dc.subjectdrug stability
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmedicinal plant
dc.subjectoxidation
dc.subjectPiperaceae
dc.subjectplant leaf
dc.subjectPothomorphe umbellata
dc.titleAntioxidant and cytotoxic effects of crude extract, fractions and 4-nerolidylcathecol from aerial parts of pothomorphe umbellata L. (Piperaceae)en
dc.typeArtigo
dcterms.licensehttp://www.hindawi.com/journals/aaa/guidelines/
unesp.author.lattes4787521613038315[6]
unesp.author.orcid0000-0003-0757-7876[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Filosofia e Ciências, Maríliapt

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