Atividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula) frente ao herpersvírus bovino tipo 1 (BoHV-1) e ao vírus da diarreia viral bovina (BVDV)

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dc.contributor.authorPeter, Cristina Mendes
dc.contributor.authorPicoli, Tony
dc.contributor.authorZani, João Luiz
dc.contributor.authorLatosinski, Giulia Soares [UNESP]
dc.contributor.authorde Lima, Marcelo
dc.contributor.authorVargas, Gilberto Dávila
dc.contributor.authorHübner, Silvia de Oliveira
dc.contributor.authorFischer, Geferson
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:15:42Z
dc.date.available2018-12-11T17:15:42Z
dc.date.issued2017-07-01
dc.description.abstractAmong the biological properties of propolis, the antimicrobial activity has received prominent attention. In this paper, we describe the antiviral and virucidal effect of three hydroalcoholic extracts of propolis (brown, green and jataí bees (Tetragonisca angustula), against bovine herpesvirus type-1 (BoHV-1) and bovine viral diarrhea Virus (BVDV). All hydroalcoholic extracts were obtained from ethanol extraction. The chemical composition of propolis extracts was determined by high-performance liquid chromatography coupled to mass spectrometer (UFLC-PDA-ESI-TOF/MS) to identify and quantify compounds such as caffeic acid and p-coumaric acid, chlorogenic acid, ferulic, and flavonoids such as rutin. Cell toxicity and antiviral activity of propolis extracts in monolayers of MDBK cells (Madin- Darby Bovine Kidney) were assessed by microscopic observation and quantified by the MTT assay (3- (4.5 dimethylthiazol-2yl) -2- 5-diphenyl-2H-tetrazolato bromine). Propolis extract from Jataí bees proved to be less cytotoxic (1.57mg / ml) when compared to green extracts (0.78mg / ml) and brown (0.39mg/mL). Regarding antiviral activity, propolis has shown greater efficacy in both cellular treatments (post and pre-exposure) against BoHV- 1 when compared to other extracts, ie, there was increased cell viability compared to cell and virus controls. Extracts from Jataí showed activity against both viruses (BoHV-1 and BVDV) infection in the pre-test, whereas brown propolis demonstrated action only against the BoHV-1 in the pre-infection method. To determine the virucidal activity, it were used different dilutions of virus, as well as different temperatures and incubation times. The green propolis at 37°C led to a greater reduction in viral titer (4.33log) compared to brown (3.5log) and jataí (3.24log). Jataí propolis showed the best results in both temperatures (22°C esand 37°C) when tested against BVDV. In summary, the evaluated extracts showed antiviral and virucidal activity against BoHV-1 and BVDV, and may be important targets for the development of new compounds as an alternative to commercial antivirals.pt
dc.description.affiliationUniversidade Federal de Pelotas (UFPel), Campus Capão do Leao, Avenida Eliseu Maciel s/n
dc.description.affiliationDepartamento de Veterinária Preventiva UFPel, Campus Capão do Leao, Avenida Eliseu Maciel s/n
dc.description.affiliationDepartamento de Higiene Veterinária e Saúde Pública (DHVSP) Faculdade de Medicina Veterinária e Zootecnia (FMVZ) Universidade Estadual Paulista Julio de Mesquita Filho (Unesp) Campus de Botucatu, CP 560, Rua Prof. Doutor Walter Mauricio Correa s/n
dc.description.affiliationUnespDepartamento de Higiene Veterinária e Saúde Pública (DHVSP) Faculdade de Medicina Veterinária e Zootecnia (FMVZ) Universidade Estadual Paulista Julio de Mesquita Filho (Unesp) Campus de Botucatu, CP 560, Rua Prof. Doutor Walter Mauricio Correa s/n
dc.format.extent667-675
dc.identifierhttp://dx.doi.org/10.1590/s0100-736x2017000700003
dc.identifier.citationPesquisa Veterinaria Brasileira, v. 37, n. 7, p. 667-675, 2017.
dc.identifier.doi10.1590/s0100-736x2017000700003
dc.identifier.fileS0100-736X2017000700667.pdf
dc.identifier.issn1678-5150
dc.identifier.issn0100-736X
dc.identifier.scieloS0100-736X2017000700667
dc.identifier.scopus2-s2.0-85032504584
dc.identifier.urihttp://hdl.handle.net/11449/175409
dc.language.isoeng
dc.language.isopor
dc.relation.ispartofPesquisa Veterinaria Brasileira
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAlternative treatment
dc.subjectAntiviral activity
dc.subjectBees
dc.subjectBoHV-1
dc.subjectBovine herpesvirus type-1
dc.subjectBovine Viral Diarrhea Virus
dc.subjectBVDV
dc.subjectChemical analysis
dc.subjectCytotoxicity
dc.subjectHydroalcoholic extracts
dc.subjectPropolis
dc.subjectTetragonisca angustula
dc.subjectVirucidal activity
dc.titleAtividade antiviral e virucida de extratos hidroalcoólicos de própolis marrom, verde e de abelhas Jataí (Tetragonisca angustula) frente ao herpersvírus bovino tipo 1 (BoHV-1) e ao vírus da diarreia viral bovina (BVDV)pt
dc.title.alternativeAntiviral and virucidal activity of hydroalcoholic extracts of propolis brown, green and jataí bees (Tetragonisca angustula) against Bovine Herpesvirus Type-1 (BoHV-1) and Bovine Viral Diarrhea Virus (BVDV)en
dc.typeArtigo

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