Immunocytochemical localization of heparin in secretory granules of rat peritoneal mast cells using a monoclonal anti-heparin antibody (ST-1)

Página do item simplificado
dc.contributor.authorOliani, S. M.
dc.contributor.authorFreymuller, E.
dc.contributor.authorTakahashi, H. K.
dc.contributor.authorStraus, A. H.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:21:15Z
dc.date.available2014-05-20T15:21:15Z
dc.date.issued1997-02-01
dc.description.abstractWe performed immunogold labeling with an ST-1 monoclonal antibody (IgM), specific for intact heparin, to define the subcellular localization of heparin in mast cells. Rat peritoneal mast cells were fixed by a modified Karnovsky method and embedded in Araldite. Ultrathin sections were first treated with sodium periodate and then sequentially incubated with MAb ST-1, rabbit anti-mouse IgM, and protein A-gold. By transmission electron microscopy, gold particles were localized inside cytoplasmic granules of peritoneal mast cells. In contrast, with the same procedure, no labeling was observed in mast cells from rat intestinal mucosa. Control sections of rat peritoneal or intestinal mucosa mast Mast cells cells treated with an irrelevant MAb (IgM) did not show any labeling. Treatment with nitrous Heparin acid abolished the reactivity of MAb ST-1 with peritoneal mast cells. These results Granules show that different mast cells can be identified regarding their heparin content by immunochemical procedures using MAb ST-1.en
dc.description.affiliationUNIV FED SAO PAULO,EPM,DEPT BIOCHEM,BR-04023900 SAO PAULO,BRAZIL
dc.description.affiliationUNIV FED SAO PAULO,EPM,ELECTRON MICROSCOPY UNIT,BR-04023900 SAO PAULO,BRAZIL
dc.description.affiliationUNESP,IBILCE,DEPT BIOL,S JOSE RIO PR,BRAZIL
dc.description.affiliationUnespUNESP,IBILCE,DEPT BIOL,S JOSE RIO PR,BRAZIL
dc.format.extent231-235
dc.identifierhttp://dx.doi.org/10.1177/002215549704500208
dc.identifier.citationJournal of Histochemistry & Cytochemistry. Seattle: Histochemical Soc Inc., v. 45, n. 2, p. 231-235, 1997.
dc.identifier.doi10.1177/002215549704500208
dc.identifier.issn0022-1554
dc.identifier.lattes5102737730539655
dc.identifier.urihttp://hdl.handle.net/11449/32410
dc.identifier.wosWOS:A1997WM75700008
dc.language.isoeng
dc.publisherHistochemical Soc Inc
dc.relation.ispartofJournal of Histochemistry & Cytochemistry
dc.relation.ispartofjcr2.816
dc.relation.ispartofsjr1,344
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectMast cellspt
dc.subjectheparinpt
dc.subjectgranulespt
dc.subjectimmunolabelingpt
dc.subjectmonoclonal antibodypt
dc.subjectelectron microscopypt
dc.titleImmunocytochemical localization of heparin in secretory granules of rat peritoneal mast cells using a monoclonal anti-heparin antibody (ST-1)en
dc.typeArtigo
dcterms.licensehttp://www.sagepub.com/authors/journal/permissions.sp#7
dcterms.rightsHolderHistochemical Soc Inc
unesp.author.lattes5102737730539655
unesp.author.orcid0000-0003-0918-2130[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Biologia - IBILCE