A putative role of teneurin-2 and its related proteins in astrocytes

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dc.contributor.authorTessarin, Gestter W.L. [UNESP]
dc.contributor.authorMichalec, Ola M.
dc.contributor.authorTorres-Da-Silva, Kelly R. [UNESP]
dc.contributor.authorDa Silva, André V. [UNESP]
dc.contributor.authorCruz-Rizzolo, Roelf J. [UNESP]
dc.contributor.authorGonçalves, Alaide [UNESP]
dc.contributor.authorGasparini, Daniele C. [UNESP]
dc.contributor.authorHorta-Júnior, José A.C. [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorBittencourt, Jackson C.
dc.contributor.authorLovejoy, David A.
dc.contributor.authorCasatti, Cláudio A. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Toronto
dc.contributor.institutionUniversidade Federal de Mato Grosso do Sul (UFMS)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2019-10-06T16:38:24Z
dc.date.available2019-10-06T16:38:24Z
dc.date.issued2019-01-01
dc.description.abstractTeneurins are type II transmembrane proteins comprised of four phylogenetically conserved homologs (Ten-1-4) that are highly expressed during neurogenesis. An additional bioactive peptide named teneurin C-terminal-associated peptide (TCAP-1-4) is present at the carboxyl terminal of teneurins. The possible correlation between the Ten/TCAP system and brain injuries has not been explored yet. Thus, this study examined the expression of these proteins in the cerebral cortex after mechanical brain injury. Adult rats were subjected to cerebral cortex injury by needle-insertion lesion and sacrificed at various time points. This was followed by analysis of the lesion area by immunohistochemistry and conventional RT-PCR techniques. Control animals (no brain injury) showed only discrete Ten-2-like immunoreactive pyramidal neurons in the cerebral cortex. In contrast, Ten-2 immunoreactivity was significantly up-regulated in the reactive astrocytes in all brain-injured groups (p < 0.0001) when compared to the control group. Interestingly, reactive astrocytes also showed intense immunoreactivity to LPHN-1, an endogenous receptor for the Ten-2 splice variant named Lasso. Semi-quantitative analysis of Ten-2 and TCAP-2 expression revealed significant increases of both at 48 h, 3 days and 5 days (p < 0.0001) after brain injury compared to the remaining groups. Immortalized cerebellar astrocytes were also evaluated for Ten/TCAP expression and intracellular calcium signaling by fluorescence microscopy after TCAP-1 treatment. Immortalized astrocytes expressed additional Ten/TCAP homologs and exhibited significant increases in intracellular calcium concentrations after TCAP-1 treatment. This study is the first to demonstrate that Ten-2/TCAP-2 and LPHN-1 are upregulated in reactive astrocytes after a mechanical brain injury. Immortalized cerebellar astrocytes expressed Ten/TCAP homologs and TCAP-1 treatment stimulated intracellular calcium signaling. These findings disclose a new functional role of the Ten/TCAP system in astrocytes during tissue repair of the CNS.en
dc.description.affiliationDepartment of Basic Sciences School of Dentistry of Araçatuba São Paulo State University (UNESP)
dc.description.affiliationDepartment of Anatomy Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.affiliationDepartment of Cell and Systems Biology University of Toronto
dc.description.affiliationSchool of Medicine Federal University of Mato Grosso do Sul (UFMS)
dc.description.affiliationDepartment of Anatomy Institute of Biomedical Sciences São Paulo University (USP)
dc.description.affiliationUnespDepartment of Basic Sciences School of Dentistry of Araçatuba São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Anatomy Institute of Biosciences of Botucatu São Paulo State University (UNESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdFAPESP: 2008/02771-6
dc.description.sponsorshipIdFAPESP: 2009/54141-9
dc.description.sponsorshipIdFAPESP: 2010/52068-0
dc.description.sponsorshipIdFAPESP: 2012/03067-6
dc.description.sponsorshipIdFAPESP: 2012/08833-9
dc.description.sponsorshipIdFAPESP: 2013/26779-4
dc.identifierhttp://dx.doi.org/10.3389/fnins.2019.00655
dc.identifier.citationFrontiers in Neuroscience, v. 13, n. JUN, 2019.
dc.identifier.doi10.3389/fnins.2019.00655
dc.identifier.issn1662-453X
dc.identifier.issn1662-4548
dc.identifier.lattes4408095517346846
dc.identifier.lattes8487462626931877
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.orcid0000-0001-5650-7343
dc.identifier.scopus2-s2.0-85068466489
dc.identifier.urihttp://hdl.handle.net/11449/189369
dc.language.isoeng
dc.relation.ispartofFrontiers in Neuroscience
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAdult rat
dc.subjectCerebral cortex
dc.subjectLatrophilin
dc.subjectMechanical brain injury
dc.subjectReactive astrocytes
dc.subjectTeneurin
dc.subjectTeneurin c-terminal associated peptide
dc.titleA putative role of teneurin-2 and its related proteins in astrocytesen
dc.typeArtigo
unesp.author.lattes4408095517346846[9]
unesp.author.lattes8487462626931877[12]
unesp.author.orcid0000-0003-4859-0583[9]
unesp.author.orcid0000-0001-5650-7343[12]

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