Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples

Página do item simplificado
dc.contributor.authorde Oliveira, Luciana Bonome Zeminian [UNESP]
dc.contributor.authorDella Coletta, Amanda Manoel [UNESP]
dc.contributor.authorGardizani, Taiane Priscila [UNESP]
dc.contributor.authorGarces, Hans Garcia [UNESP]
dc.contributor.authorBagagli, Eduardo [UNESP]
dc.contributor.authorTrilles, Luciana
dc.contributor.authorBarrozo, Ligia Vizeu
dc.contributor.authorMarques, Sílvio de Alencar [UNESP]
dc.contributor.authorFaveri, Julio De [UNESP]
dc.contributor.authorDias-Melicio, Luciane Alarcão [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionOswaldo Cruz Foundation (FIOCRUZ)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T13:48:55Z
dc.date.available2023-07-29T13:48:55Z
dc.date.issued2023-03-01
dc.description.abstractParacoccidioidomycosis (PCM) is the main and most prevalent systemic mycosis in Latin America, that until recently, it was believed to be caused only by Paracoccidioides brasiliensis (P. brasiliensis). In 2006, researchers described three cryptic species: S1, PS2, PS3, and later, another one, PS4. In 2009, Paracoccidioides lutzii (Pb01-like) was described, and in 2017, a new nomenclature was proposed for the different agents: P. brasiliensis (S1), P. americana (PS2), P. restrepiensis (PS3), and P. venezuelensis (PS4). These species are not uniformly distributed throughout Latin America and, knowing that more than one cryptic species could coexist in some regions, we aimed to identify those species in patients’ biopsy samples for a better understanding of the distribution and occurrence of these recently described species in Botucatu region. The Hospital of Medical School of Botucatu—UNESP, which is a PCM study pole, is located in São Paulo State mid-west region and is classified as a PCM endemic area. Genotyping analyses of clinical specimens from these patients that have been diagnosed and treated in our Hospital could favor a possible correlation between genetic groups and mycological and clinical characteristics. For this, molecular techniques to differentiate Paracoccidioides species in these biopsies, such as DNA extraction, PCR, and sequencing of three target genes (ITS, CHS2, and ARF) were conducted. All the sequences were analyzed at BLAST to testify the presence of P. brasiliensis. The phylogenetic trees were constructed using Mega 7.0 software and showed that 100% of our positive samples were from S1 cryptic species, therefore P. brasiliensis. This is important data, demonstrating the predominance of this species in the São Paulo State region.en
dc.description.affiliationLaboratory of Immunopathology and Infectious Agents—LIAI UNIPEX—Experimental Research Unity—Sector 5 Medical School of Botucatu São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), SP
dc.description.affiliationEvandro Chagas National Institute of Infectious Disease Oswaldo Cruz Foundation (FIOCRUZ), RJ
dc.description.affiliationDepartment of Geography School of Philosophy Literature and Human Sciences University of São Paulo (USP), SP
dc.description.affiliationDepartment of Dermatology Medical School of Botucatu São Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Pathology Medical School of Botucatu São Paulo State University (UNESP), SP
dc.description.affiliationUnespLaboratory of Immunopathology and Infectious Agents—LIAI UNIPEX—Experimental Research Unity—Sector 5 Medical School of Botucatu São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Dermatology Medical School of Botucatu São Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Pathology Medical School of Botucatu São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.3390/microorganisms11030562
dc.identifier.citationMicroorganisms, v. 11, n. 3, 2023.
dc.identifier.doi10.3390/microorganisms11030562
dc.identifier.issn2076-2607
dc.identifier.scopus2-s2.0-85151464384
dc.identifier.urihttp://hdl.handle.net/11449/248614
dc.language.isoeng
dc.relation.ispartofMicroorganisms
dc.sourceScopus
dc.subjectmolecular biology
dc.subjectmolecular phylogenetic analysis
dc.subjectparacoccidiodomycosis
dc.subjectParacoccidioides brasiliensis
dc.subjectParacoccidioides species complex
dc.titleMolecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samplesen
dc.typeArtigo
unesp.author.orcid0000-0002-6100-3534[4]
unesp.author.orcid0000-0002-8003-4109[5]
unesp.author.orcid0000-0001-7322-6789[7]
unesp.author.orcid0000-0001-9254-2074[10]

Arquivos

Coleções

Artigos - Patologia - FMB
Artigos - Dermatologia e Radioterapia - FMB