Citrus aurantium L. essential oil exhibits anxiolytic-like activity mediated by 5-HT1A-receptors and reduces cholesterol after repeated oral treatment

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dc.contributor.authorCosta, Celso A R A [UNESP]
dc.contributor.authorCury, Thaís C. [UNESP]
dc.contributor.authorCassettari, Bruna O. [UNESP]
dc.contributor.authorTakahira, Regina K. [UNESP]
dc.contributor.authorFlório, Jorge C.
dc.contributor.authorCosta, Mirtes [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-27T11:28:33Z
dc.date.available2014-05-27T11:28:33Z
dc.date.issued2013-02-23
dc.description.abstractBackground: The current treatments for anxiety disorders and depression have multiple adverse effects in addition to a delayed onset of action, which has prompted efforts to find new substances with potential activity in these disorders. Citrus aurantium was chosen based on ethnopharmacological data because traditional medicine refers to the Citrus genus as useful in diminishing the symptoms of anxiety or insomnia, and C. aurantium has more recently been proposed as an adjuvant for antidepressants. In the present work, we investigated the biological activity underlying the anxiolytic and antidepressant effects of C. aurantium essential oil (EO), the putative mechanism of the anxiolytic-like effect, and the neurochemical changes in specific brain structures of mice after acute treatment. We also monitored the mice for possible signs of toxicity after a 14-day treatment.Methods: The anxiolytic-like activity of the EO was investigated in a light/dark box, and the antidepressant activity was investigated in a forced swim test. Flumazenil, a competitive antagonist of benzodiazepine binding, and the selective 5-HT1A receptor antagonist WAY100635 were used in the experimental procedures to determine the mechanism of action of the EO. To exclude false positive results due to motor impairment, the mice were submitted to the rotarod test.Results: The data suggest that the anxiolytic-like activity observed in the light/dark box procedure after acute (5 mg/kg) or 14-day repeated (1 mg/kg/day) dosing was mediated by the serotonergic system (5-HT1A receptors). Acute treatment with the EO showed no activity in the forced swim test, which is sensitive to antidepressants. A neurochemical evaluation showed no alterations in neurotransmitter levels in the cortex, the striatum, the pons, and the hypothalamus. Furthermore, no locomotor impairment or signs of toxicity or biochemical changes, except a reduction in cholesterol levels, were observed after treatment with the EO.Conclusion: This work contributes to a better understanding of the biological activity of C. aurantium EO by characterizing the mechanism of action underlying its anxiolytic-like activity. © 2013 Costa et al; licensee BioMed Central Ltd.en
dc.description.affiliationDepartment of Pharmacology Institute of Biosciences Unesp - Univ Estadual Paulista, P.O. Box 51018618-970, Botucatu, São Paulo
dc.description.affiliationDepartment of Pathology, School of Veterinary Medicine and Animal Science Unesp - Univ Estadual Paulista Laboratório Clínico Veterinário, 18618-970, Botucatu, SP
dc.description.affiliationDepartment of Pathology School of Veterinary Medicine USP - University of São Paulo, Av. Orlando Marques de Paiva, 87, São Paulo, SP
dc.description.affiliationUnespDepartment of Pharmacology Institute of Biosciences Unesp - Univ Estadual Paulista, P.O. Box 51018618-970, Botucatu, São Paulo
dc.description.affiliationUnespDepartment of Pathology, School of Veterinary Medicine and Animal Science Unesp - Univ Estadual Paulista Laboratório Clínico Veterinário, 18618-970, Botucatu, SP
dc.identifierhttp://dx.doi.org/10.1186/1472-6882-13-42
dc.identifier.citationBMC Complementary and Alternative Medicine, v. 13.
dc.identifier.doi10.1186/1472-6882-13-42
dc.identifier.file2-s2.0-84874044564.pdf
dc.identifier.issn1472-6882
dc.identifier.orcid0000-0003-3323-4199
dc.identifier.scopus2-s2.0-84874044564
dc.identifier.urihttp://hdl.handle.net/11449/74626
dc.identifier.wosWOS:000315656000001
dc.language.isoeng
dc.relation.ispartofBMC Complementary and Alternative Medicine
dc.relation.ispartofjcr2.109
dc.relation.ispartofsjr0,858
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAnxiolytic
dc.subjectCitrus aurantium
dc.subjectEssential oil
dc.subjectMice
dc.subjectNeurochemistry
dc.subjectSerotonin
dc.subjectbeta pinene
dc.subjectcholesterol
dc.subjectdiazepam
dc.subjectessential oil
dc.subjectflumazenil
dc.subjectlimonene
dc.subjectmyrcene
dc.subjectn [2 [4 (2 methoxyphenyl) 1 piperazinyl]ethyl] n (2 pyridyl)cyclohexanecarboxamide
dc.subjectserotonin 1A receptor
dc.subjectsour orange extract
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectantidepressant activity
dc.subjectbrain cortex
dc.subjectchemical composition
dc.subjectcholesterol blood level
dc.subjectclinical assessment tool
dc.subjectconcentration (parameters)
dc.subjectcontrolled study
dc.subjectcorpus striatum
dc.subjectfalse positive result
dc.subjectforced swimming test
dc.subjecthypothalamus
dc.subjectlight dark box test
dc.subjectlocomotion
dc.subjectmale
dc.subjectmotor dysfunction
dc.subjectmouse
dc.subjectneurochemistry
dc.subjectnonhuman
dc.subjectpons
dc.subjectrotarod test
dc.subjecttoxicity testing
dc.subjecttranquilizing activity
dc.subjectAnimals
dc.subjectAnti-Anxiety Agents
dc.subjectAnticholesteremic Agents
dc.subjectAnxiety
dc.subjectBehavior, Animal
dc.subjectBrain
dc.subjectCholesterol
dc.subjectCitrus
dc.subjectDepression
dc.subjectFlumazenil
dc.subjectGABA Modulators
dc.subjectLight
dc.subjectMale
dc.subjectMice, Inbred Strains
dc.subjectNeurotransmitter Agents
dc.subjectOils, Volatile
dc.subjectPhytotherapy
dc.subjectPlant Extracts
dc.subjectReceptor, Serotonin, 5-HT1A
dc.subjectRotarod Performance Test
dc.subjectSerotonin Receptor Agonists
dc.subjectSwimming
dc.titleCitrus aurantium L. essential oil exhibits anxiolytic-like activity mediated by 5-HT1A-receptors and reduces cholesterol after repeated oral treatmenten
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
unesp.author.orcid0000-0003-3323-4199[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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