Characterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira spp

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dc.contributor.authorGarcia, Leandro Encarnação [UNESP]
dc.contributor.authorde Araújo Junior, Erivelto Corrêa [UNESP]
dc.contributor.authorMelo, Larissa Martins [UNESP]
dc.contributor.authorBragato, Jaqueline Poleto [UNESP]
dc.contributor.authorPeiró, Juliana Regina [UNESP]
dc.contributor.authorFélix de Lima, Valéria Marçal [UNESP]
dc.contributor.authorMarinho, Márcia [UNESP]
dc.contributor.authorArnold, Daniel Robert [UNESP]
dc.contributor.authorLopes, Flavia Lombardi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:37:52Z
dc.date.available2018-12-11T17:37:52Z
dc.date.issued2018-07-06
dc.description.abstractLeptospirosis is a bacterial zoonosis, caused by Leptospira spp., that leads to significant morbidity and mortality worldwide. Despite considerable advances, much is yet to be discovered about disease pathogenicity. The influence of epigenetic mechanisms, particularly RNA-mediated post-transcriptional regulation of host immune response has been described following a variety of bacterial infections. The current study examined the microtranscriptome of macrophages J774A.1 following an 8h infection with virulent, attenuated and saprophyte strains of Leptospira. Microarray analysis revealed that 29 miRNAs were misregulated following leptospiral infection compared to control macrophages in a strain and virulence-specific manner. Pathway analysis for targets of these differentially expressed miRNAs suggests that several processes involved in immune response could be regulated by miRNAs. Our data provides the first evidence that host miRNAs are regulated by Leptospira infection in macrophages. A number of the identified miRNA targets participate in key immune response processes. We suggest that post-transcriptional regulation by miRNAs may play a role in host response to infection in leptospirosis.en
dc.description.affiliationDepartment of Support Production and Animal Health São Paulo State University (Unesp) School of Veterinary Medicine
dc.description.affiliationDepartment of Clinic Surgery and Animal Reproduction São Paulo State University (Unesp) School of Veterinary Medicine
dc.description.affiliationUnespDepartment of Support Production and Animal Health São Paulo State University (Unesp) School of Veterinary Medicine
dc.description.affiliationUnespDepartment of Clinic Surgery and Animal Reproduction São Paulo State University (Unesp) School of Veterinary Medicine
dc.identifierhttp://dx.doi.org/10.1371/journal.pntd.0006621
dc.identifier.citationPLoS Neglected Tropical Diseases, v. 12, n. 7, 2018.
dc.identifier.doi10.1371/journal.pntd.0006621
dc.identifier.file2-s2.0-85050725407.pdf
dc.identifier.issn1935-2735
dc.identifier.issn1935-2727
dc.identifier.lattes5071093931017493
dc.identifier.scopus2-s2.0-85050725407
dc.identifier.urihttp://hdl.handle.net/11449/180065
dc.language.isoeng
dc.relation.ispartofPLoS Neglected Tropical Diseases
dc.relation.ispartofsjr2,589
dc.relation.ispartofsjr2,589
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.titleCharacterization of the microtranscriptome of macrophages infected with virulent, attenuated and saprophyte strains of Leptospira sppen
dc.typeArtigo
unesp.author.lattes5071093931017493
unesp.author.orcid0000-0002-3173-3712[9]

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Artigos - Clínica, Cirurgia e Reprodução Animal - FMVA
Artigos - Apoio, Produção e Saúde Animal - FMVA