Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells

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dc.contributor.authorBressel, Tatiana A. B.
dc.contributor.authorDe Queiroz, Jana Dara Freires
dc.contributor.authorGomes Moreira, Susana Margarida
dc.contributor.authorDa Fonseca, Jéssyca T.
dc.contributor.authorFilho, Edson A. [UNESP]
dc.contributor.authorGuastaldi, Antônio Carlos [UNESP]
dc.contributor.authorBatistuzzo De Medeiros, Silvia Regina
dc.contributor.institutionLagoa Nova
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionPrograma de Pós Graduação em Ciências da Saúde
dc.date.accessioned2018-12-11T17:16:17Z
dc.date.available2018-12-11T17:16:17Z
dc.date.issued2017-11-28
dc.description.abstractBackground: Titanium surfaces have been modified by various approaches with the aim of improving the stimulation of osseointegration. Laser beam (Yb-YAG) treatment is a controllable and flexible approach to modifying surfaces. It creates a complex surface topography with micro and nano-scaled patterns, and an oxide layer that can improve the osseointegration of implants, increasing their usefulness as bone implant materials. Methods: Laser beam irradiation at various fluences (132, 210, or 235 J/cm2) was used to treat commercially pure titanium discs to create complex surface topographies. The titanium discs were investigated by scanning electron microscopy, X-ray diffraction, and measurement of contact angles. The surface generated at a fluence of 235 J/cm2 was used in the biological assays. The behavior of mesenchymal stem cells from an umbilical cord vein was evaluated using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, a mineralization assay, and an alkaline phosphatase activity assay and by carrying out a quantitative real-time polymerase chain reaction for osteogenic markers. CHO-k1 cells were also exposed to titanium discs in the MTT assay. Results: The best titanium surface was that produced by laser beam irradiation at 235 J/cm2 fluence. Cell proliferation analysis revealed that the CHO-k1 and mesenchymal stem cells behaved differently. The laser-processed titanium surface increased the proliferation of CHO-k1 cells, reduced the proliferation of mesenchymal stem cells, upregulated the expression of the osteogenic markers, and enhanced alkaline phosphatase activity. Conclusions: The laser-treated titanium surface modulated cellular behavior depending on the cell type, and stimulated osteogenic differentiation. This evidence supports the potential use of laser-processed titanium surfaces as bone implant materials, and their use in regenerative medicine could promote better outcomes.en
dc.description.affiliationDepartamento de Biologia Celular e Genética CB UFRN Universidade Federal Do Rio Grande Do Norte Campus Universitário Lagoa Nova
dc.description.affiliationDepartamento de Físico-Química Instituto de Química de Araraquara UNESP
dc.description.affiliationPrograma de Pós Graduação em Ciências da Saúde
dc.description.affiliationUnespDepartamento de Físico-Química Instituto de Química de Araraquara UNESP
dc.identifierhttp://dx.doi.org/10.1186/s13287-017-0717-9
dc.identifier.citationStem Cell Research and Therapy, v. 8, n. 1, 2017.
dc.identifier.doi10.1186/s13287-017-0717-9
dc.identifier.file2-s2.0-85035103138.pdf
dc.identifier.issn1757-6512
dc.identifier.scopus2-s2.0-85035103138
dc.identifier.urihttp://hdl.handle.net/11449/175555
dc.language.isoeng
dc.relation.ispartofStem Cell Research and Therapy
dc.relation.ispartofsjr1,685
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiocompatibility
dc.subjectHuman umbilical cord
dc.subjectLaser beam (Yb-YAG)
dc.subjectMesenchymal stem cells
dc.subjectOsteoinduction
dc.subjectSurface modification
dc.subjectTitanium
dc.titleLaser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cellsen
dc.typeArtigo
unesp.author.lattes6443430122330366[6]
unesp.author.orcid0000-0002-6433-3555[6]

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