Outbreak of fungemia caused by Candida parapsilosis in a neonatal intensive care unit: Molecular investigation through microsatellite analysis

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dc.contributor.authorDa Silva Ruiz, Luciana
dc.contributor.authorMontelli, Augusto Cezar [UNESP]
dc.contributor.authorSugizaki, Maria de Fátima [UNESP]
dc.contributor.authorDa Silva, Eriques Gonçalves
dc.contributor.authorDe Batista, Georgea Carla Matuura
dc.contributor.authorMoreira, Débora
dc.contributor.authorPaula, Claudete Rodrigues
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:28:45Z
dc.date.available2014-05-27T11:28:45Z
dc.date.issued2013-04-01
dc.description.abstractBackground: Opportunistic infections are an increasingly common problem in hospitals, and the yeast Candida parapsilosis has emerged as an important nosocomial pathogen, especially in neonatal intensive care units (NICUs) where it has been responsible for outbreak cases. Risk factors for C. parapsilosis infection in neonates include prematurity, very low birth weight, prolonged hospitalization, indwelling central venous catheters, hyperalimentation, intravenous fatty emulsions and broad spectrum antibiotic therapy. Molecular methods are widely used to elucidate these hospital outbreaks, establishing genetic variations among strains of yeast. Aims: The aim of this study was to detect an outbreak of C. parapsilosis in an NICU at the Hospital das Clinicas , Faculty of Medicine of Botucatu, a tertiary hospital located in São Paulo, Brazil, using the molecular genotyping by the microsatellite markers analysis. Methods: A total of 11 cases of fungemia caused by C. parapsilosis were identified during a period of 43 days in the NICU. To confirm the outbreak all strains were molecularly typed using the technique of microsatellites. Results: Out of the 11 yeast samples studied, nine showed the same genotypic profile using the technique of microsatellites. Conclusions: Our study shows that the technique of microsatellites can be useful for these purposes. In conclusion, we detected the presence of an outbreak of C. parapsilosis in the NICU of the hospital analyzed, emphasizing the importance of using molecular tools, for the early detection of hospital outbreaks, and for the introduction of effective preventive measures, especially in NICUs. © 2012 Revista Iberoamericana de Micología.en
dc.description.affiliationDepartment of Microbiology Institute of Biomedical Sciences II University of São Paulo, Av. Prof. Lineu Prestes, 1374, CEP 05508-900, São Paulo
dc.description.affiliationDepartment of Microbiology and Immunology Institute of Biosciences UNESP, Botucatu, CEP 18618000 - Botucatu, São Paulo
dc.description.affiliationUnespDepartment of Microbiology and Immunology Institute of Biosciences UNESP, Botucatu, CEP 18618000 - Botucatu, São Paulo
dc.format.extent112-115
dc.identifierhttp://dx.doi.org/10.1016/j.riam.2012.10.003
dc.identifier.citationRevista Iberoamericana de Micologia, v. 30, n. 2, p. 112-115, 2013.
dc.identifier.doi10.1016/j.riam.2012.10.003
dc.identifier.issn1130-1406
dc.identifier.issn2173-9188
dc.identifier.scopus2-s2.0-84877132610
dc.identifier.urihttp://hdl.handle.net/11449/74943
dc.identifier.wosWOS:000319530400006
dc.language.isoeng
dc.relation.ispartofRevista Iberoamericana de Micologia
dc.relation.ispartofjcr0.989
dc.relation.ispartofsjr0,432
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCandida parapsilosis
dc.subjectFungemia
dc.subjectMicrosatellite
dc.subjectNeonates
dc.subjectOutbreak
dc.subjectBrazil
dc.subjectcandidiasis
dc.subjectclinical article
dc.subjectepidemic
dc.subjectfemale
dc.subjectfungal strain
dc.subjectfungemia
dc.subjectfungus identification
dc.subjectgenotype
dc.subjecthuman
dc.subjectinfant
dc.subjectintensive care unit
dc.subjectmale
dc.subjectmicrosatellite marker
dc.subjectmolecular diagnosis
dc.subjectmolecular genetics
dc.subjectnewborn
dc.subjectnewborn intensive care
dc.subjectnonhuman
dc.subjecttertiary health care
dc.titleOutbreak of fungemia caused by Candida parapsilosis in a neonatal intensive care unit: Molecular investigation through microsatellite analysisen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
unesp.author.orcid0000-0003-2502-9021[4]
unesp.author.orcid0000-0002-1156-0834[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB