FOXE1: methylation and gene expression in canine osteosarcoma cell culture

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dc.contributor.authorAraujo, Rodolfo Françon [UNESP]
dc.contributor.authorBersano, Paulo Ricardo de Oliveira [UNESP]
dc.contributor.authorFerrasi, Adriana Camargo [UNESP]
dc.contributor.authorMontoya-Flórez, Luis Mauricio [UNESP]
dc.contributor.authorSilva, Glenda Nicioli da
dc.contributor.authorFeo, Haline Ballestero [UNESP]
dc.contributor.authorRocha, Noeme Sousa [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidad de Caldas
dc.contributor.institutionUniversidade Federal de Ouro Preto (UFOP)
dc.date.accessioned2016-07-07T12:34:28Z
dc.date.available2016-07-07T12:34:28Z
dc.date.issued2014
dc.description.abstractGene expression profiles in a canine osteosarcoma (OSA) cell culture could identify chemotherapeutic targets and sort useful from useless prognostic groups. This could allow for more personalized treatment of OSA based on an individual’s tumor expression profile. An experimental model of methylation and gene expression in canine osteosarcoma cell culture is described. Blood Samples were taken from 5 dogs with cytological and histo-pathological OSA, and one from normal bone. OSA primary cell culture was isolated and characterized. For methylation and FOXE1 expression analysis, DNA of 5x106 osteosarcoma cells was used. Results showed that about 80% OSA cell cultures sequences were methylated and 20% un-methylated. FOXE1 expression was not demonstrated in un-methylated cell culture or normal bone. The findings suggest that FOXE1 gene may be silenced in OSA cells but its absence in osteoblast cells suggests that it is not involved in the carcinogenesis of canine osteosarcoma.en
dc.description.affiliationUniversidad de Caldas Manizales, Veterinary Pathology Research Group, Faculty of Agricultural Sciences
dc.description.affiliationUniversidade Federal de Ouro Preto, Departamento de Análises Clínicas, Escola de Farmácia
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia de Botucatu
dc.description.affiliationUnespUniversidade Estadual Paulista, Departamento de Patologia, Faculdade de Medicina de Botucatu
dc.format.extent293-302
dc.identifierhttp://onljvetres.com/foxeabs2014.htm
dc.identifier.citationOnline Journal of Veterinary Research, v. 18, n. 3, p. 293-302, 2014.
dc.identifier.issn1328-925X
dc.identifier.lattes6077735918469284
dc.identifier.lattes3587895085226224
dc.identifier.orcid0000-0001-9200-5391
dc.identifier.orcid0000-0002-8188-8149
dc.identifier.urihttp://hdl.handle.net/11449/140567
dc.language.isoeng
dc.relation.ispartofOnline Journal of Veterinary Research
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectImmunocytochemicalen
dc.subjectEpigeneticen
dc.subjectOsteoblasten
dc.subjectFOXE1 gene expressionen
dc.titleFOXE1: methylation and gene expression in canine osteosarcoma cell cultureen
dc.typeArtigo
unesp.author.lattes6077735918469284
unesp.author.lattes3587895085226224[3]
unesp.author.orcid0000-0001-9200-5391[3]
unesp.author.orcid0000-0002-8188-8149
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt
unesp.departmentClínica Veterináriapt
unesp.departmentPatologiapt

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Artigos - Clínica Veterinária - FMVZ
Artigos - Patologia - FMB