Anti-clastogenic effect of beta-glucan extracted from barley towards chemically induced DNA damage in rodent cells

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dc.contributor.authorAngeli, J. P. F.
dc.contributor.authorRibeiro, L. R.
dc.contributor.authorBellini, M. F.
dc.contributor.authorMantovani, M. S.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:54:21Z
dc.date.available2014-05-20T13:54:21Z
dc.date.issued2006-06-01
dc.description.abstractbeta-Glucan (BG) was tested in vitro to determine its potential clastogenic and/or anti-clastogenic activity, and attempts were made to elucidate its possible mechanism of action by using combinations with an inhibitor of DNA polymerase. The study was carried out on cells deficient (CHO-k1) and cells proficient (HTC) in phases I and II enzymes, and the DNA damage was assessed by the chromosomal aberration assay. BG did not show a clastogenic effect, but was anti-clastogenic in both cell lines used, and at all concentrations tested (2.5, 5 and 10 mg/mL) in combination with damage inducing agents (methylmethane sulfonate in cell line CHO-k1, and methylmethane sulfonate or 2-aminoanthracene in cell line HTC). BG also showed a protective effect in the presence of a DNA polymerase beta inhibitor (cytosine arabinoside-3-phosphate, Ara-C), demonstrating that BG does not act through an anti-mutagenic mechanism of action involving DNA polymerase beta.en
dc.description.affiliationUniv Estadual Londrina, Dept Biol Geral, CCB, BR-86051990 Londrina, Parana, Brazil
dc.description.affiliationUNESP, Dept Biol Geral, Rio Claro, SP, Brazil
dc.description.affiliationUNESP, Dept Biol Celular & Mol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol Geral, Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Biol Celular & Mol, Sao Jose do Rio Preto, SP, Brazil
dc.format.extent319-324
dc.identifierhttp://dx.doi.org/10.1191/0960327106ht631oa
dc.identifier.citationHuman & Experimental Toxicology. London: Hodder Arnold, Hodder Headline Plc, v. 25, n. 6, p. 319-324, 2006.
dc.identifier.doi10.1191/0960327106ht631oa
dc.identifier.issn0960-3271
dc.identifier.urihttp://hdl.handle.net/11449/19426
dc.identifier.wosWOS:000238439300005
dc.language.isoeng
dc.publisherHodder Arnold, Hodder Headline Plc
dc.relation.ispartofHuman & Experimental Toxicology
dc.relation.ispartofjcr1.840
dc.relation.ispartofsjr0,559
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectanti-mutagenesispt
dc.subjectbeta-glucanspt
dc.subjectchromosomal aberrationspt
dc.subjectdrug-metabolizing cellspt
dc.titleAnti-clastogenic effect of beta-glucan extracted from barley towards chemically induced DNA damage in rodent cellsen
dc.typeArtigo
dcterms.licensehttp://www.uk.sagepub.com/aboutus/openaccess.htm
dcterms.rightsHolderHodder Arnold, Hodder Headline Plc
unesp.author.orcid0000-0001-5268-6508[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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