Utilização do método de aglutina ção direta e da reação de imunofluorescência indireta na detecção de anticorpos para Toxoplasma gondii em cavalos naturalmente infectados

Página do item simplificado
dc.contributor.authorLangoni, Hélio [UNESP]
dc.contributor.authorda Silva, Aristeu Vieirada
dc.contributor.authorPezerico, Sandia Bergamaschi [UNESP]
dc.contributor.authorde Lima, Vanessa Yuride [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Paranaense
dc.date.accessioned2014-05-27T11:22:44Z
dc.date.available2014-05-27T11:22:44Z
dc.date.issued2007-12-01
dc.description.abstractInfection by Toxoplasma gondii in equines is usually not apparent, it being characterized by presence of antibody titers and tissue cysts. This study was aimed at verifying the presence of anti-Toxoplasma antibodies in equine serum by modified agglutination test (MAT) and reaction to indirect immunofluorescent antibody test (IFAT). 1984 samples of serum were examined, by MAT, using whole formalin fixed tachyzoites of T.gondii as antigen. The samples reacting in the MAT test, and 150 other negative samples, chosen at random, were also tested by IFAT, utilizing anti-equine IgG. The association among the test results was verified by the McNemar test. 138 samples were positive in the MAT test, with 60 (46.38%) presenting reaction at a dilution of 1:64; 52 (37.7%) at 1:256; 19 (13.8%) at 1:1024; five (3.6%) at 1:4096; and two (1.45%) at 1:16384. Of 132 positive MAT samples, 14 were negative in the IFAT test, but the statistical analysis indicated general agreement in results of the tests. The results obtained showed agreement among the tests utilized, and the possibility of participation of equines in the transmission of toxoplasmosis to carnivorous animals, and also to humans.en
dc.description.affiliationNúcleo de Pesquisa em Zoonoses Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu - SP
dc.description.affiliationMestrado em Ciência Animal Universidade Paranaense, Umuarama - PR
dc.description.affiliationUnespNúcleo de Pesquisa em Zoonoses Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista, Botucatu - SP
dc.format.extent27-32
dc.identifierhttp://www.revistas.usp.br/bjvras/article/view/26657
dc.identifier.citationBrazilian Journal of Veterinary Research and Animal Science, v. 44, n. 1, p. 27-32, 2007.
dc.identifier.file2-s2.0-77950445237.pdf
dc.identifier.issn1413-9596
dc.identifier.issn1678-4456
dc.identifier.lattes5326072118518067
dc.identifier.scopus2-s2.0-77950445237
dc.identifier.urihttp://hdl.handle.net/11449/70192
dc.language.isopor
dc.relation.ispartofBrazilian Journal of Veterinary Research and Animal Science
dc.relation.ispartofsjr0,225
dc.relation.ispartofsjr0,225
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectEquine
dc.subjectIFAT
dc.subjectMAT
dc.subjectSerology
dc.subjectToxoplasma gondii
dc.subjectAnimalia
dc.subjectEquidae
dc.subjectToxoplasma
dc.titleUtilização do método de aglutina ção direta e da reação de imunofluorescência indireta na detecção de anticorpos para Toxoplasma gondii em cavalos naturalmente infectadospt
dc.title.alternativeUtilization of modified agglutination test and indirect immunofluorescent antibody test for the detection of Toxoplasma gondii antibodies in naturally exposed horsesen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/bjvras/paboutj.htm
unesp.author.lattes5326072118518067
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-77950445237.pdf
Tamanho:
197.67 KB
Formato:
Adobe Portable Document Format
Baixar

Coleções

Artigos - Higiene Veterinária e Saúde Pública - FMVZ