Development of twenty-one polymorphic microsatellite markers for the fungus-growing ant, Mycocepurus goeldii (Formicidae: Attini), using Illumina paired-end genomic sequencing

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dc.contributor.authorRabeling, Christian
dc.contributor.authorLove, Cara N.
dc.contributor.authorLance, Stacey L.
dc.contributor.authorJones, Kenneth L.
dc.contributor.authorPierce, Naomi E.
dc.contributor.authorBacci, Maurício [UNESP]
dc.contributor.institutionHarvard Univ
dc.contributor.institutionUniv Georgia
dc.contributor.institutionUniv Colorado
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-03-18T15:53:10Z
dc.date.available2015-03-18T15:53:10Z
dc.date.issued2014-09-01
dc.description.abstractObligate social parasites, or inquilines, exploit the colonies of free-living social species and evolved at least 80 times in ants alone. Most species of the highly specialized inquiline social parasites are rare, only known from one or very few, geographically isolated populations, and the sexual offspring of most inquiline species mates inside the maternal colony. Therefore, inquiline populations are believed to be small and genetically homogeneous due to inbreeding. To comparatively study the genetic diversity of the socially parasitic fungus-growing ant, Mycocepurus castrator, and its only known host species, Mycocepurus goeldii, and to infer the parasite's conservation status, we developed 21 microsatellite markers for the host species, M. goeldii, and evaluated whether these markers cross-amplify in the social parasite, M. castrator. We isolated and characterized a total of 21 microsatellite loci for M. goeldii. The loci were screened for 24 individuals from geographically distant and genetically divergent populations in Brazil. The number of alleles per locus ranged from 18 to 4, the observed heterozygosity ranged from 0.25 to 0.636, and the probability of identity values ranged from 0.011 to 0.146. Preliminary analyses show that these markers cross amplify in the closely related social parasite species M. castrator. These newly developed loci provide tools for studying the genetic diversity and the evolution of social parasitism in the Mycocepurus host-parasite system.en
dc.description.affiliationHarvard Univ, Museum Comparat Zool, Cambridge, MA 02138 USA
dc.description.affiliationUniv Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA
dc.description.affiliationUniv Colorado, Dept Biochem & Mol Genet, Sch Med, Aurora, CO 80045 USA
dc.description.affiliationUNESP Univ Estadual Paulista, Inst Biociencias, Ctr Estudos Insetos Sociais, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUNESP Univ Estadual Paulista, Inst Biociencias, Ctr Estudos Insetos Sociais, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipHarvard Society
dc.description.sponsorshipWilliam F. Milton Fund
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipPROPE-UNESP fund
dc.description.sponsorshipNSF
dc.description.sponsorshipDOE
dc.description.sponsorshipIdFAPESP: 11/50226-0
dc.description.sponsorshipIdNSFSES-0750480
dc.description.sponsorshipIdDOEDE-FC09-07SR22506
dc.format.extent739-741
dc.identifierhttp://dx.doi.org/10.1007/s12686-014-0204-x
dc.identifier.citationConservation Genetics Resources. Dordrecht: Springer, v. 6, n. 3, p. 739-741, 2014.
dc.identifier.doi10.1007/s12686-014-0204-x
dc.identifier.issn1877-7252
dc.identifier.urihttp://hdl.handle.net/11449/116380
dc.identifier.wosWOS:000340404900066
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofConservation Genetics Resources
dc.relation.ispartofjcr0.742
dc.relation.ispartofsjr0,430
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPolygynyen
dc.subjectPolyandryen
dc.subjectSocial parasitismen
dc.subjectInquilinismen
dc.subjectPAL_FINDERen
dc.subjectSSRen
dc.titleDevelopment of twenty-one polymorphic microsatellite markers for the fungus-growing ant, Mycocepurus goeldii (Formicidae: Attini), using Illumina paired-end genomic sequencingen
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.orcid0000-0002-5619-1411[6]

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Rio Claro - CEIS - Centro de Estudos de Insetos Sociais