Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs

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dc.contributor.authorAmbrósio, DL [UNESP]
dc.contributor.authorSilva, MTA [UNESP]
dc.contributor.authorCicarelli, RMB [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:25:21Z
dc.date.available2014-05-20T13:25:21Z
dc.date.issued2007-02-01
dc.description.abstractSmall nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in splicing, catalyzed by spliceosomes, large RNA-protein complexes composed of various snRNPs. Even though they are well defined in mammals, snRNPs are still not totally characterized in certain trypanosomatids as Trypanosoma cruzi. For this reason we subjected snRNAs (U2, U4, U5, and U6) from T. cruzi epimastigotes to molecular characterization by polymerase chain reaction (PCR) and reverse transcription-PCR. These amplified sequences were cloned, sequenced, and compared with those other of trypanosomatids. Among these snRNAs, U5 was less conserved and U6 the most conserved. Their respective secondary structures were predicted and compared with known T. brucei structures. In addition, the copy number of each snRNA in the T. cruzi genome was characterized by Southern blotting.en
dc.description.affiliationUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências Biológicas
dc.description.affiliationUnespUniversidade Estadual Paulista Faculdade de Ciências Farmacêuticas Departamento de Ciências Biológicas
dc.format.extent97-106
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762007000100017
dc.identifier.citationMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 102, n. 1, p. 97-106, 2007.
dc.identifier.doi10.1590/S0074-02762007000100017
dc.identifier.fileS0074-02762007000100017.pdf
dc.identifier.issn0074-0276
dc.identifier.scieloS0074-02762007000100017
dc.identifier.urihttp://hdl.handle.net/11449/8021
dc.language.isoeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.relation.ispartofjcr2.833
dc.relation.ispartofsjr1,172
dc.rights.accessRightsAcesso aberto
dc.sourceSciELO
dc.subjectTrypanosoma cruzien
dc.subjectribonucleoproteinen
dc.subjectsmall nuclear RNPen
dc.subjectsmall nuclear RNAen
dc.subjectspliceosomeen
dc.subjecttrans-splicingen
dc.titleCloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAsen
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Ciências Biológicas - FCFAR