Histological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolis

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dc.contributor.authorConceição, Mariana [UNESP]
dc.contributor.authorGushiken, Lucas Fernando Sérgio [UNESP]
dc.contributor.authorAldana-Mejía, Jennyfer Andrea
dc.contributor.authorTanimoto, Matheus Hikaru
dc.contributor.authorFerreira, Marcos Vital de Sá [UNESP]
dc.contributor.authorAlves, Andreia Cristina Miranda [UNESP]
dc.contributor.authorMiyashita, Marina Naomi [UNESP]
dc.contributor.authorBastos, Jairo Kenupp
dc.contributor.authorBeserra, Fernando Pereira [UNESP]
dc.contributor.authorPellizzon, Cláudia Helena [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2023-07-29T16:07:58Z
dc.date.available2023-07-29T16:07:58Z
dc.date.issued2022-11-01
dc.description.abstractSkin wound healing is a complex process that requires the mutual work of cellular and molecular agents to promote tissue restoration. In order to improve such a process, especially in cases of impaired healing (e.g., diabetic ulcer, chronic wounds), there is a search for substances with healing properties and low toxicity: two features that some natural products—such as the bee product named propolis—exhibit. Propolis is a resinous substance obtained from plant resins and exudates with antioxidant, anti-inflammatory, and antitumoral activities, among other biological ones. Based on the previously reported healing actions of different types of propolis, the Brazilian red propolis (BRP) was tested for this matter. A skin wound excision model in male Wistar rats was performed using two topical formulations with 1% red propolis as treatments: hydroalcoholic extract and Paste. Macroscopical, histological and immunohistochemical analysis were performed, revealing that red propolis enhanced wound contraction, epithelialization, reduced crust formation, and modulated the distribution of healing associated factors, mainly collagen I, collagen III, MMP-9, TGF-β3 and VEGF. Biochemical analysis with the antioxidants SOD, MPO, GSH and GR showed that propolis acts similarly to the positive control, collagenase, increasing these molecules’ activity. These results suggest that BRP promotes enhanced wound healing by modulating growth factors and antioxidant molecules related to cutaneous wound healing.en
dc.description.affiliationInstitute of Biosciences São Paulo State University (UNESP), SP
dc.description.affiliationSchool of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo (USP), SP
dc.description.affiliationUnespInstitute of Biosciences São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.3390/antiox11112188
dc.identifier.citationAntioxidants, v. 11, n. 11, 2022.
dc.identifier.doi10.3390/antiox11112188
dc.identifier.issn2076-3921
dc.identifier.scopus2-s2.0-85149485447
dc.identifier.urihttp://hdl.handle.net/11449/249739
dc.language.isoeng
dc.relation.ispartofAntioxidants
dc.sourceScopus
dc.subjectantioxidants
dc.subjectbee products
dc.subjectBrazilian red propolis
dc.subjecthistopathology
dc.subjectskin wound healing
dc.titleHistological, Immunohistochemical and Antioxidant Analysis of Skin Wound Healing Influenced by the Topical Application of Brazilian Red Propolisen
dc.typeArtigo
unesp.author.orcid0000-0003-0736-1899[3]
unesp.author.orcid0000-0002-1063-3026[4]
unesp.author.orcid0000-0002-0498-4444[9]

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