Screening of Saccharum spp. genotypes for sugarcane yellow leaf virus resistance by combining symptom phenotyping and highly precise virus titration

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dc.contributor.authorBurbano, Roberto C.V. [UNESP]
dc.contributor.authorGonçalves, Marcos C.
dc.contributor.authorNobile, Paula M.
dc.contributor.authordos Anjos, Ivan A.
dc.contributor.authorda Silva, Marcel F.
dc.contributor.authorPerecin, Dilermando [UNESP]
dc.contributor.authorAnjos, Luciana O.S.
dc.contributor.authorFernandes, Vivian B.V.R.
dc.contributor.authorPinto, Luciana R.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCentro de Sanidade Vegetal
dc.contributor.institutionCentro de Cana
dc.contributor.institutionEscuela Superior Politécnica Del Litoral
dc.date.accessioned2021-06-25T11:12:21Z
dc.date.available2021-06-25T11:12:21Z
dc.date.issued2021-06-01
dc.description.abstractYellow leaf disease (YLD), caused by sugarcane yellow leaf virus (SCYLV), has been reported to infect sugarcane worldwide causing significant yield losses and is considered a major disease of this crop. A panel composed of 98 genotypes encompassing basic germplasm, commercial cultivars, and elite clones was assayed in the nursery and a replicated field for resistance to SCYLV, combining symptom expression and virus quantification. Virus symptom intensity was evaluated using a diagrammatic scale while virus titer was estimated by DAS-ELISA (Double Antibody Sandwich-ELISA) and RT-qPCR (Reverse Transcription Quantitative PCR) in the nursery and field trials, respectively. Resistance was evaluated alongside symptom development kinetics. Based on the symptom intensity, 52 (53.06%) genotypes were classified as resistant in the nursery and 42 (42.86%) in the field. Twenty-nine (29.59%) genotypes showed no symptoms in the nursery and field trial. Moderately resistant genotypes showed a low correlation between virus titer and symptom intensity. The kinetics of symptom development increased over time in moderately susceptible and susceptible genotypes. The SCYLV incidence assessed by RT-qPCR was 92.55%, which was detected in 83% of the asymptomatic genotypes. The broad-sense heritability based on symptom expression and relative quantification was 52.62% and 68%, respectively. The virus quantification assays allowed for the identification of potential genotypes immune to SCYLV.en
dc.description.affiliationUniversidade Estadual Paulista UNESP, Jaboticabal
dc.description.affiliationInstituto Biológico Centro de Sanidade Vegetal, São Paulo
dc.description.affiliationInstituto Agronômico Centro de Cana, Ribeirão Preto
dc.description.affiliationEscuela Superior Politécnica Del Litoral
dc.description.affiliationUnespUniversidade Estadual Paulista UNESP, Jaboticabal
dc.description.sponsorshipSecretaría de Educación Superior, Ciencia, Tecnología e Innovación
dc.identifierhttp://dx.doi.org/10.1016/j.cropro.2021.105577
dc.identifier.citationCrop Protection, v. 144.
dc.identifier.doi10.1016/j.cropro.2021.105577
dc.identifier.issn0261-2194
dc.identifier.scopus2-s2.0-85101411426
dc.identifier.urihttp://hdl.handle.net/11449/208447
dc.language.isoeng
dc.relation.ispartofCrop Protection
dc.sourceScopus
dc.subjectDAS-ELISA
dc.subjectPhenotyping
dc.subjectPolerovirus
dc.subjectRT-qPCR
dc.subjectSugarcane
dc.subjectYellow leaf disease
dc.titleScreening of Saccharum spp. genotypes for sugarcane yellow leaf virus resistance by combining symptom phenotyping and highly precise virus titrationen
dc.typeArtigo

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