Heparin-binding proteins of canine seminal plasma

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dc.contributor.authorSouza, Fabiana Ferreira de [UNESP]
dc.contributor.authorMello Martins, Maria Isabel
dc.contributor.authordos Santos Fernandes, Carlos Eurico
dc.contributor.authorMartins Ribolla, Paulo Eduardo
dc.contributor.authorLopes, Maria Denise [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCtr Univ Rio Preto
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.date.accessioned2014-02-26T17:26:41Z
dc.date.accessioned2014-05-20T13:41:45Z
dc.date.available2014-02-26T17:26:41Z
dc.date.available2014-05-20T13:41:45Z
dc.date.issued2006-10-01
dc.description.abstractHeparin-binding proteins (HBP) from seminal plasma have been expected to participate in modulation of the acrosomal reaction, and have been correlated with fertility in some species. However, they have not been described in the dog. The aim of this study was to document the HBPs of canine seminal plasma. Six pooled samples of seminal plasma from three crossbred dogs were used. The HBPs were isolated by heparin affinity chromatography and the fractions recovered were pooled. One-dimensional sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) was carried out on 12 and 18% vertical minigels. The stained gels were scanned and the molecular weight (kDa) values for each band within a lane were calculated by image analysis software. The electrophoresis analysis of the pooled eluded fractions identified 19 bands, with molecular weights varying from 61.5 to 5.2 kDa. Previous studies, using one-dimensional SDS-PAGE, identified two bands (67 and 58.6 kDa), which were positively correlated with some semen parameters (sperm motility, sperm vigor, percentage of morphologically normal sperm and plasma membrane integrity). The 61.5 kDa band detected in the present study apparently corresponded to the 58.6 kDa band identified previously. Canine seminal plasma contained HBP; since HBP modulate the acrosome reaction in other species, they may have the same function in the dog. Further studies are necessary to better characterize this protein and determine if it is associated with fertility in the dog. (c) 2006 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, FMVZ, Dept Reprod Anim & Radiol Vet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationCtr Univ Rio Preto, UNIRP, Lab Reprod Anim, Hosp Vet, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUEL, Dept Clin Vet, Londrina, PR, Brazil
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Dept Parasitol, Inst Biociencias, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, FMVZ, Dept Reprod Anim & Radiol Vet, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Dept Parasitol, Inst Biociencias, Botucatu, SP, Brazil
dc.format.extent1606-1609
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2006.02.016
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 66, n. 6-7, p. 1606-1609, 2006.
dc.identifier.doi10.1016/j.theriogenology.2006.02.016
dc.identifier.issn0093-691X
dc.identifier.lattes3577149748456880
dc.identifier.lattes6666129914663018
dc.identifier.urihttp://hdl.handle.net/11449/14493
dc.identifier.wosWOS:000240887300037
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectheparin-binding proteinspt
dc.subjectseminal plasmapt
dc.subjectdogpt
dc.subjectcaninept
dc.titleHeparin-binding proteins of canine seminal plasmaen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes3577149748456880
unesp.author.lattes6666129914663018
unesp.author.orcid0000-0003-4721-1801[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Reprodução Animal e Radiologia Veterinária - FMVZ
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