Polymorphisms of miR-196a2 (rs11614913) and miR-605 (rs2043556) confer susceptibility to gastric cancer

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dc.contributor.authorPoltronieri-Oliveira, Ayla Blanco [UNESP]
dc.contributor.authorMadeira, Fernanda Fernandez [UNESP]
dc.contributor.authorNunes, Denis Bruno Santos Marques [UNESP]
dc.contributor.authorRodrigues, Gabriela Helena [UNESP]
dc.contributor.authorLopes, Beatriz Camargo [UNESP]
dc.contributor.authorManoel-Caetano, Fernanda S. [UNESP]
dc.contributor.authorBiselli, Joice Matos [UNESP]
dc.contributor.authorSilva, Ana Elizabete [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:32:18Z
dc.date.available2018-12-11T17:32:18Z
dc.date.issued2017-06-01
dc.description.abstractBackground and purpose MicroRNAs (miRNAs) have been appointed as potential biomarkers for gastric cancer. Recent evidences suggest that single-nucleotide polymorphisms (SNPs) in miRNAs may change the miRNA biogenesis and influence cancer susceptibility. The aim of this study was to investigate the association of Mir-SNPs in miR-27a rs895819, miR-125a rs12976445, miR-196a2 rs11614913, miR-499 rs3746444, and miR-605 rs2043556 and their effect, alone and combined, on gastric cancer risk. Methods DNA samples obtained from 151 gastric cancer (GC) patients and 249 non-cancer subjects (control) were genotyped using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique or the TaqMan® SNP Genotyping Assay. Multiple logistic regression analysis was used to assess the associations between the miRNA polymorphisms and GC risk according to log-additive, dominant, and recessive models. Combined genotype analyses were also performed, using Fisher's exact test. Results The Mir-SNPs miR-27a rs895819, miR-125a rs12976445 and miR-499 rs3746444 were not associated with risk of GC. However, the miR-196a2 rs11614913 TT variant genotype was associated with a significantly increased risk for GC in the recessive model (OR = 2.88; 95% CI = 1.45–5.72; P = 0.002), and miR-605 rs2043556 AG and GG genotype carriers showed a significantly higher risk for GC in both the dominant (adjusted OR = 1.79; 95% CI = 1.14–2.82; P = 0.001) and the log-additive models (OR = 1.46; 95% CI = 1.01–2.12; P = 0.044). The combined genotype analysis showed that the presence of the three risk genotypes miR-27a G/miR-125a C/miR-605 G is associated with higher risk of GC (OR = 11.00; 95% CI = 1.13–106.5; P = 0.046). In addition, the combined genotype analysis of only miR-196a2 rs11614913 and miR-605 rs2043556 revealed that individuals carrying both risk alleles (miR-196a2 T/miR-605 G) also presented a significantly higher risk for GC compared to double wild-type homozygous individuals (OR = 2.00; 95% CI = 1.08–3.71; P = 0.031). Conclusions Our data showed that miR-196a2 rs11614913 and miR-605 rs2043556, alone or in combination, modulate the risk of developing GC in a Brazilian population, and that the combined genotypes miR-27a G/miR-125a C/miR-605 G may potentiate this risk.en
dc.description.affiliationCytogenetics and Molecular Biology Laboratory Department of Biology UNESP Univ. Estadual Paulista, Rua Cristovão Colombo, 2265
dc.description.affiliationUnespCytogenetics and Molecular Biology Laboratory Department of Biology UNESP Univ. Estadual Paulista, Rua Cristovão Colombo, 2265
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/15036-8
dc.format.extent154-163
dc.identifierhttp://dx.doi.org/10.1016/j.genrep.2017.04.006
dc.identifier.citationGene Reports, v. 7, p. 154-163.
dc.identifier.doi10.1016/j.genrep.2017.04.006
dc.identifier.issn2352-4065
dc.identifier.issn2452-0144
dc.identifier.scopus2-s2.0-85018556128
dc.identifier.urihttp://hdl.handle.net/11449/178835
dc.language.isoeng
dc.relation.ispartofGene Reports
dc.relation.ispartofsjr0,165
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCancer risk
dc.subjectGastric cancer
dc.subjectGenotyping
dc.subjectMicroRNAs
dc.subjectPolymorphisms
dc.titlePolymorphisms of miR-196a2 (rs11614913) and miR-605 (rs2043556) confer susceptibility to gastric canceren
dc.typeArtigo
unesp.author.lattes2503906319038306[8]
unesp.author.orcid0000-0003-1491-8878[8]

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