Molecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern brazil

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dc.contributor.authorMongruel, Anna Cláudia Baumel
dc.contributor.authorIkeda, Priscila [UNESP]
dc.contributor.authorde Sousa, Keyla Carstens Marques [UNESP]
dc.contributor.authorBenevenute, Jyan Lucas [UNESP]
dc.contributor.authorFalbo, Margarete Kimie
dc.contributor.authorMachado, Rosangela Zacarias [UNESP]
dc.contributor.authorCarrasco, Adriano de Oliveira Torres
dc.contributor.authorAndré, Marcos Rogério [UNESP]
dc.contributor.authorSeki, Meire Christina
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual do Centro-Oeste – UNICENTRO
dc.date.accessioned2019-10-06T16:08:44Z
dc.date.available2019-10-06T16:08:44Z
dc.date.issued2018-10-01
dc.description.abstractArthropod-borne pathogens are medically important because of their ability to cause diseases in their hosts. The purpose of this study was to detect the occurrence of Ehrlichia spp., piroplasmids and Hepatozoon spp. in dogs with anemia and thrombocytopenia in southern Brazil. EDTA-whole blood was collected from 75 domestic dogs presenting anemia or/and thrombocytopenia from Guarapuava, state of Paraná, Brazil. DNA samples were subjected to conventional PCR assays for Ehrlichia spp. (dsb), piroplasmids (18S rRNA) and Hepatozoon spp. (18S rRNA), followed by sequencing and phylogenetic analyses. Among the 75 dogs, one (1.33%) was positive for Hepatozoon sp. and six (8%) were positive for piroplasmids in 18S rRNA cPCR assays. None of the dogs showed positive results in Ehrlichia spp.-cPCR targeting dsb gene. The phylogenetic analyses revealed that three piroplasm sequences were clustered with Rangellia vitalii, while one sequence was grouped with B. vogeli. The only sequence obtained from Hepatozoon spp.-PCR protocol was pooled with H. canis. Therefore, there is urgent need for differential molecular diagnosis of the two piroplasm species cited as etiological agents in clinical cases of canine hemoparasitic diseases, given the higher pathogenic potential of R. vitalii than of B. vogeli.en
dc.description.affiliationLaboratório de Zoonoses e Epidemiologia Molecular Universidade Federal do Paraná – UFPR
dc.description.affiliationLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista – UNESP
dc.description.affiliationLaboratório de Patologia Clínica Departamento de Medicina Veterinária Universidade Estadual do Centro-Oeste – UNICENTRO
dc.description.affiliationLaboratório de Doenças Infecciosas e Parasitárias Departamento de Medicina Veterinária Universidade Estadual do Centro-Oeste – UNICENTRO
dc.description.affiliationUnespLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista – UNESP
dc.format.extent505-513
dc.identifierhttp://dx.doi.org/10.1590/s1984-296120180069
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria, v. 27, n. 4, p. 505-513, 2018.
dc.identifier.doi10.1590/s1984-296120180069
dc.identifier.fileS1984-29612018000400505.pdf
dc.identifier.issn0103-846X
dc.identifier.scieloS1984-29612018000400505
dc.identifier.scopus2-s2.0-85057986508
dc.identifier.urihttp://hdl.handle.net/11449/188459
dc.language.isoeng
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinaria
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBabesia vogeli
dc.subjectHepatozoon canis
dc.subjectPhylogenetic analysis
dc.subjectRangelia vitalii
dc.subjectTick borne diseases
dc.titleMolecular detection of vector borne pathogens in anemic and thrombocytopenic dogs in southern brazilen
dc.titleDetecção molecular de patógenos transmitidos por vetores em cães anemicos e trombocitopenicos no Sul do Brasilpt
dc.typeArtigo

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